骨组织工程的.pdf

·骨组织工程 · 网孔纳米羟基磷灰石 /聚酰胺人工骨 修复兔桡骨缺损 孟纯阳 　安洪 　蒋电明 　李玉宝 　　【摘要 】　目的 　探讨新型网孔纳米羟基磷灰石 /聚酰胺 66 ( n - HA /PA66)复合材料作为骨组 织工程支架修复长骨骨缺损的能力及作为人工骨修复替代材料的可行性。　方法 　选用新西兰 大白兔双侧桡骨制作骨缺损模型 ,将 n - HA /PA66复合材料植入左侧骨缺损处 ,右侧骨缺损以脱 蛋白骨 ( dep roteinel bone, DPB)植入作为实验对照 ,另做不植入任何材料的骨缺损空白对照 ,同时 设置未行任何处理只供力学测试的正常对照。在 2, 4, 8, 12, 16周各时相点分别进行大体观察、X 线照片、组织学切片、扫描电镜观察及力学测试。　结果 　 n - HA /PA66人工骨组与牛脱蛋白骨 ( bDPB)组骨缺损均完全修复 ,而空白对照组骨缺损未见修复 ; n - HA /PA66与 bDPB两组间及两 组分别与正常对照组生物力学各项测试指标比较 ,差异均无统计学意义 ( P 0. 05)。　结论 　新 型骨修复和重建材料 n - HA /PA66具有良好的骨传导能力和力学特性以及生物相容性 ,有望成为 骨组织工程中修复骨缺损的理想支架材料。 【关键词 】　羟基磷灰石 ; 　聚酰胺 ; 　复合材料 ; 　骨缺损 Repa ir of bone defect w ith porous com posite of nano2hydroxyapa tite and polym ide　　M ENG Chun2yang, AN Hong, J IANG D ian2m ing, L I Yu2bao. D epartm ent of O rthopaed ics, F irst A ff ilia ted Hospi2 ta l of Chongqing U niversity of M edical Sciences, Chongqing 400016, ChinaCorrespond ing author: 蒋电 明 , 电话 : 023 - Em ail: jdm 571026@ vip. 163. com 【Abstract】　O bjective　 To evaluate the ability of porous nano2hydroapatite crystals and polyam2 ide composite ( n2HA /PA66) in repairing bone defects and discuss the feasibility of n2HA /PA66 as bone substitute material. 　M ethods 　The animal models of bilateral radius bone defect created by surgery in the New Zealand white rabbits were imp lanted with n2HA /PA66 on the left bone defect ( experimental group) and with dep roteined bone (DPB) on the right defects ( control group). The bone defects with no imp lant were used as blank control group. Normal control was set in rabbits that received nothing of treat2 ment. The effect was observed by gross, X2ray, histopathological, transm ission electron m icroscop ic and dynam ic exam inations 2, 4, 6, 8, 12 weeks after operation. In the meantime, the interface of imp lants and recep tor bone was observed by the scanning electron m icroscope and biomechanics determ ined 16 weeks after operation. 　Results 　 The defects of experimental group and experimental control group got repaired perfec