Shanxi Codonopsis different growth years of the RP-HPLC fingerprint of_0.docVIP

 PAGE \* MERGEFORMAT 19 Shanxi Codonopsis different growth years of the RP-HPLC fingerprint of Of: Xiu-Wen Wang, Zhaohui Hui, Liu Yang Qing, Liu Xiaofang, Cheng Ruomin, Genius [Abstract] Objective To study the different growth years Shanxi fingerprints of Radix Codonopsis, Radix Codonopsis for scientific evaluation of the intrinsic quality of available information. Method using RP-HPLC method, column: GraceSmart RP18 (250 mm × 4.6 mm, 5 μm), to acetonitrile -0.1% acetic acid as mobile phase, flow rate 0.6 mL / min, detection wavelength was 268 nm, column temperature was 30 ℃. the results of HPLC fingerprint of Radix Codonopsis samples showed significantly different samples of different ages Codonopsis and similarity, precision degree of stability and reproducibility of RSD were “3%, with acetylene glycoside Codonopsis Codonopsis can be recognized as a reference sample of the active ingredient. Conclusion The method is simple, reliable, and the use of the integrity of fingerprint , Shanxi Codonopsis medicines can provide a useful reference for quality control. [Keywords:] Codonopsis, different growth years, Codonopsis acetylene glycosides, fingerprints, HPLC Abstract: Objective Chromatography fingerprint of Radix Codonopsis of different age from Shanxi province was studied to provide the reference for the science appraisal of intrinsic quality of Radix codonopsis. Method A RP-HPLC method was applied on GraceSmart-RP18 (250 mm × 4.6 mm, 5 μm) column with acetonitrile-0.1% acetic acid gradient elution as mobile phase, and the flow-rate was 0.6 mL / min. The detection wavelength was at 268 nm and the column temperature was at 30 ℃. Result Chromatography fingerprint of Radix Codonopsis can demonstrate obviously the difference and the similarity of Radix Codonopsis of different age. The RSD were lower than 3% in the precision, stability and the repetitiveness trial, and the codonopsis pilosula alkyne glucoside can be used as the reference to identify the act