Study on quality standard drug capsules.docVIP

 PAGE \* MERGEFORMAT 19 Study on quality standard drug capsules Authors: Jin Yi-ran, DU Ying-feng, Xu Huijun, Wei Guo, Yuan Zhifang, Zhang Tong [Abstract] Objective To establish quality standards for drug capsules. Thin Layer Chromatography (TLC) method of drug capsules in the honeysuckle, red sage root, skullcap, gardenia, white peony root, Ginseng, Rehmannia qualitative identification; using high performance liquid chromatography (HPLC) method of preparation of the Green acid for determination. Chromatographic conditions: analysis using C18 column and acetonitrile -0.4% phosphoric acid (twelve eighty-eight) as mobile phase, detection wavelength of 327 nm, injection volume of 20 μ l. The results of thin-layer chromatography for the test materials and control solution chromatography in medicinal or reference substance the same color appear on the corresponding position of the spots, negative control without interference; high performance liquid chromatography, chlorogenic acid 1.82 ~ 9.10 μ g * ml-1 the scope of a good linear relationship (r = 0.999 7), the average recovery was 99.09%. Conclusion TLC spots showing clear, specific, can be used for identification of drug capsule; high-performance liquid chromatography method is simple, rapid, high precision, accuracy, and can be used for drug quality control. [Keywords:] drug capsule; thin-layer chromatography; high-performance liquid chromatography; chlorogenic acid Abstract: ObjectiveTo establish the quality standard for Jiedu Capsules.MethodsFlos Lonicerae, Radix Salviae Miltiorrhizae, Radix Scutellarine, Fructus Gardeniae, Radix Paeoniae Alba, Radix Ginseng and Radix Rehmanniae were identified using TLC.The content for chlorogenic acid in Jiedu Capsules was determined with HPLC . HPLC analytical method was carried out using a C18 column and a mixture containing 12 volume of acetonitrile and 88 volume of 0.4% phosphoric acid solution as the mobile phase.The detection wavelength was set at 327 nm.ResultsSp