T sulindac increased cell killing mechanism of gastric cancer cells.docVIP

 PAGE \* MERGEFORMAT 16 T sulindac increased cell killing mechanism of gastric cancer cells Of: the right to Liu Jun, Chen revival, New Gong, Zhang Baofu, Zhang Song, Lvxiao Ting, Zheng Tao, Li Yi, Juan [Abstract] Objective To investigate the cytotoxicity of sulindac increased ???T the mechanism of gastric cancer cells. T cell culture method of conventional methods, collecting the first 9 days of ???T cultured cells with different concentrations of sulindac (1,2,4,8,16 , 32 mol / L) induced 24 h, collected supernatants for cytokine -interferon (IFN-), tumor necrosis factor-@ (TNF-@) and interleukin 12 (IL-12) detected precipitation cells for perforin, granzyme B, NKG2D flow cytometry and the use of lactate dehydrogenase (LDH) release assay ???T cell killing cancer cells (SGC-7901 and BCG-823) activity. each test sample and indicators are located not increase drug-induced group. Results sulindac concentration 8 mol / L when, ???T cell perforin and granzyme B reached the maximum (respectively (71.5 + -5.3)% and (78.3 + -7.1) %), significantly higher (respectively, (51.4 + -7.3)% and (60.9 + -7.1)%). ???T cells were treated with sulindac at different concentrations in culture supernatants after the induction of IL-12 and TNF- @ concentrations did not differ; sulindac at 8 mol / L when the concentration of IFN- in supernatant reached the highest ((246.1 + -20.7) ng / L), significantly higher ((196.1 + -13.2) ng / L); when sulindac concentration of 32 mol / L when, ???T cells of 3 cytokines were significantly lower than the control group, both had significant differences (P lt;0.05). ???T cells induced by sulindac Anti-SGC-7901 cells and BCG-823 activity in 8 mol / L and reached the peak, this time on the SGC-7901 and the killing activity of BCG-823 were (73.6 + -3.9)% and (76.3 + -3.9)%, with the control group ((60.1 + -3.7)% and (59.2 + -4.5)%) there were significant difference (P lt;0.05). Conclusion ???T sulindac induced SGC-7901 cell killing and BCG-82