The duration of intake of pomegranate juice on the aging anti-oxidant function in mice.docVIP

 PAGE \* MERGEFORMAT 13 The duration of intake of pomegranate juice on the aging anti-oxidant function in mice [Abstract] of pomegranate juice on mice aging antioxidant. Methods 72 aged mice, male and female in half, the animals were randomly divided into high-dose group, low-dose group and the control group, the daily dose group were in accordance with 20? ML / kg pomegranate juice, 10? ML / kg pomegranate juice fed to the control group by 20? mL / kg distilled water gavage. 1,2,3,4 weeks, respectively, the end of the first to take six mice in each group pull the eye to take blood and dislocated killed, 3? 500? R / min centrifugation 15? Min after the separation of serum, immediately installed in eppendorf tube, Purchase -20? ℃ to save stand-by determination of total antioxidant capacity (T  AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH  PX), malondialdehyde (MDA); and the liver rapidly frozen for T  AOC, SOD, GSH  PX, MDA were determined. Results Compared with control group, the first two weeks of high-dose group of mice and the control groups of mice total antioxidant capacity was no significant difference in the first 3 weeks the difference between the two began to have statistical significance. High-dose group and low dose group mice serum and liver total antioxidant capacity, SOD and GSH  PX activity significantly increased; and decreased serum and liver MDA was not obvious. The role of high-dose group were significantly lower doses. Conclusion pomegranate juice has good anti-oxidation function, as ingestion of pomegranate juice, the extension of time, aging, anti-oxidant levels of mouse body gradually. [Keywords:] pomegranate total antioxidant capacity of superoxide dismutase glutathione peroxidase MDA different times on aging mice Abstract: Objective To explore the anti  oxidation effect of pomegranate juice on aging mice. Methods 72 aging mice, half female and half male, were randomly divided into three groups: the hi