Wolfberry RAPD Reaction System and Optimization.docVIP

 PAGE \* MERGEFORMAT 19 Wolfberry RAPD Reaction System and Optimization Author: Yan Feng Kun, XU Xing, Min Yin, Yang Yaya, Wang Na 【Abstract】 Objective To explore the Chinese wolfberry RAPD reaction system and its optimization. Methods commonly used in our laboratory RAPD reaction system based on the RAPD amplification of genomic DNA results to determine the best Chinese wolfberry RAPD reaction system. Resulted in the creation of the RAPD reaction system suitable for Chinese wolfberry: 20μl system containing 1 × PCR buffer, 50 ng template, 0.4 μM 10 bp random primers, 1UTaq DNA polymerase, 2 mM Mg2, 125μM dNTPs. Conclusion Wolfberry RAPD reaction system can be used for the RAPD analysis of Chinese wolfberry. Keywords: Chinese wolfberry; RAPD; reaction system Establishment and Optimization of RAPD Reaction System in Lycium barbarum Abstract: ObjectiveTo study the RAPD reaction system in Lycium barbarum L. and optimization of it. MethodsBased on the RAPD reaction system was often used in our lab, RAPD reaction system was tested by the result of RAPD. ResultsAn RAPD reaction system suitable for Lycium barbarum L. was established, that was, 20μl amplification containing 1 × PCR buffer, 50ng template DNA, 0.4μm primer, 1UTaq DNA polymerase, 2 mmol / L Mg2, 125 μmol / L dNTPs. ConclusionThe RAPD reaction system can be used to RAPD analysis in Lycium barbarum. Keywords:: Lycium barbarum; RAPD; Reaction system RAPD by Williams and the Welsh two research groups almost simultaneously in 1990, built a molecular marker techniques [1,2], is currently the most commonly used traditional Chinese medicine researchers DNA marker technology, but the interference of RAPD vulnerable to a variety of factors The optimum amplification conditions are different in different species, and therefore of the wolfberry to RAPD analysis, it is absolutely necessary for the various factors to optimize the reaction conditions, to establish a stabl