With the hepatitis B virus X gene microarray protein carboxy-terminal 40 amino acid deletion of differentially expressed genes.docVIP

 PAGE \* MERGEFORMAT 22 With the hepatitis B virus X gene microarray protein carboxy-terminal 40 amino acid deletion of differentially expressed genes 【Abstract】 Objective cDNA microarray screening full-length sequence of hepatitis B virus X protein, X protein, carboxy-terminal deletion of 40 amino acid sequence of differentially expressed genes between the initial HBV-related hepatocellular carcinoma to explore the molecular mechanism. Methods of 20 228 cDNA spots with the point-like instrument on a specially designed glass slide was prepared by cDNA microarray, the hepatitis B virus HBx protein with full-length gene sequences and HBx protein carboxy-terminal deletion of 40 amino acids (HBx3 ‘ 40) gene sequence of the pcDNA3 () plasmid were transfected hepatoma cell line Huh  7, two cells, the total extracted RNA, reverse transcription of cRNA, using Cys3 and Cy5 respectively, two kinds of fluorescent tags, and expression microarray hybridization, by computer scanning and data processing, filtering out the differentially expressed genes. The results of differentially expressed genes are 167, including 145 up-regulated genes, down-regulated genes 22, including the proto-oncogene and tumor suppressor genes, cell cycle and apoptosis-related genes, signal transduction and immune regulation-related genes, cell proliferation and metabolism and gene transcription and other related coding genes. Conclusions using cDNA microarray analysis of gene expression differences, can quickly filter out HBx and HBx3 ‘ 40 differentially expressed genes and between the efficient study of gene function, in order to further study the carcinogenic mechanism of HBV provides a theoretical basis for . Keywords: cDNA microarray; HBV X protein; deletion; gene; differential expression Identification of Differentially Expressed Genes in Hepatitis B Virus X Protein C  terminally Truncated 40 Amino Acids by cDNA Microarray Abstract: Objective To investigate th