Velcade-induced multiple myeloma cell line U266 Cell Apoptosis.docVIP

 PAGE \* MERGEFORMAT 21 Velcade-induced multiple myeloma cell line U266 Cell Apoptosis Authors: Li-Juan Chen, Jian-Yong Li, Si-Xuan Qian, Zhu Guangrong, ZHENG Wen-juan 【Abstract】 In order to study velcade-induced multiple myeloma cell line U266 cell apoptosis effects and mechanism, using trypan blue dye to detect 2,10,50, and 100 nmol / L velcade deal U266 cell viability, using flow cytometry Cytometric Analysis Annexin-V, mitochondrial transmembrane potential (Δψm) and oxygen free radicals (ROS), using semi-quantitative RT-PCR to detect bcl-2 mRNA expression. The results showed that: velcade inhibit U266 cell proliferation;-induced apoptosis in U266 cells, 24-hour proportion of Annexin-V positive cells increased, △ ψM reduced; 12 hours was significantly higher when the reactive oxygen species, the fluorescence intensity enhancement; anti-apoptosis gene bcl-2 expression reduced . Conclusion: velcade on U266 cells growth inhibition and cytotoxicity, which may be through the endogenous pathway of apoptosis induced U266 cell apoptosis. Keywords: velcade; multiple myeloma; apoptosis Multiple Myeloma Cell Line U266 Apoptosis Induced by Velcade Abstract To investigate the effect of velcade on multiple myeloma cell line U266 apoptosis and its mechanism, cell viability was estimated by trypan blue dye exclusion. Annexin-V, mitochondrial transmembrane potential (Δψm) and reactive oxygen species (ROS) labeled by DCFHDA were examined by flow cytometry, the expression of bcl-2 mRNA was detected by semi-quatitative RT-PCR. The results showed that the velcade inhibited the growth of U266 cells and reduced cell viability accompanied by appearance of morphologic characteristics of apoptosis. Velcade at 50 nmol / L increased Annexin V positivity and fluorescence intensity of DCF because of ROS generation while it decreased the Δψm of U266 cells. Expression of anti-apoptotic gene bcl-2 mRNA also decreased. It is concluded that velcade inhibite th