羊肚菌菌塘土壤细菌群落的结构及多样性.PDFVIP

湖南农业大学学报(自然科学版) 2015 年 8 月 第 41 卷 第 4 期 Journal of Hunan Agricultural University (Natural Sciences), Aug. 2015, 41(4):428–434 DOI:10.13331/ki.jhau.2015.04.017 投稿网址： 羊肚菌菌塘土壤细菌群落的结构及多样性 熊川 1，李小林 2，李强 1，郑林用 1,3* (1.四川大学生命科学学院，四川 成都 610065；2.四川省农业科学院土壤肥料研究所，四川 成都 610066；3.四川 省农业科学院，四川 成都 610066) ? 摘 要：为了解野生羊肚菌的生境，研究其菌塘土壤细菌群落的结构与多样性，运用 PCR–DGGE 技术，对采集 于凉山州冕宁县野生羊肚菌发生地的 8 个羊肚菌菌塘土样和 2 个非菌塘土样进行分析。结果表明：羊肚菌菌塘土 壤与非菌塘土壤细菌群落的结构差异和多样性差异较大；菌塘土壤的多样性指数(H)和丰度(S)比非菌塘土壤的高； 菌塘土壤中 Thermosporotrichaceae、Myxococcaceae 和 Rhodospirillaceae 等 3 科细菌在羊肚菌菌塘土壤中占据优势 地位；羊肚菌菌塘土壤细菌多样性程度总体优于非菌塘土壤。 关 键 词：羊肚菌；土壤；细菌；群落结构 中图分类号：S154.38+1 文献标志码：A 文章编号：1007?1032(2015)04?0428?07 Bacteria community structure and diversity in Morchella colonies Xiong Chuan1, Li Xiaolin2, Li Qiang1，Zheng Linyong1,3* (1.College of Life Science, Sichuan University, Chengdu 610065, China; 2. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 3. Sichuan Academy of Agricultural Sciences, Chengdu 610066, China) Abstract: Ten soil samples were collected from various sites in Mianning, Liangshan, Sichuan province to investigate the Morchella habitat and know about the bacteria community structure and diversity in Morchella colonies by using the approach of denaturing gradient gel electrophoresis (DGGE). According to the microbial community in these samples, the bacteria community structures varied from soil samples. The diversity index (H) and abundance (S) in morels soil were higher than those of in non-morels soil. There were three sorts of prevailing microbes, they were thermosporotrich