PI3K p85α蛋白表达缺失对SW480细胞侵袭及转移的影响.pdfVIP

2640 实用医学杂志 2013 年第 29 卷第 16 期 PI3K p85α 蛋白表达缺失对 SW480 细胞侵袭及 转移的影响 孙娟郭升超 摘要 目的:探讨 RNA 干扰靶向抑制 PI3K p85α 的表达对人大肠癌 SW480 细胞侵袭及转移的影响。方 法:构建慢病毒PI3K p85α 千扰载体并转染 SW480 勿施，通过如j 痕实验、Trans附II 实验及参附实验，明确 PI3K p85α 表达变化对大肠癌细胞侵袭及转移的影响。结果:划痕实验结采显示低表达凹3K p85α 的大肠癌 细胞愈合能力下降;Transwell 实验显示低表达贝3Kp85α 的大肠癌细胞迁移及侵袭能力下降;参附实验显示 低表达 P13K p85α 的大肠癌细胞秘附能力降低。结论:PI3K p85α 蛋白表达缺失可明显抑制大肠癌细胞株 SW480 侵袭及转移能力，P13K p85α 可能成为大肠癌基因治疗约新靶点。 关键词 PI3K p85α; RNA 千扰; 大肠癌; 转移 Effect of PI3K p85αdepletion 00 the invasion and metastasis of SW480 cells SUN Yan , GUO Sheng-chω. Department of Ga.s troentemlogy , the Third Affil四ed Hospit a1 ofG目angzhou Medic a1 College , Guangzhou 510150 , China [Abstract) Objective To investigate the effect of RNA interference targeting PI3K p85αexpression inhibition on the invasion and metastasis of human colorectal cancer cell line SW480. Methods Lentivirus interference ca时ers were constructed and transfected inlo SW480 cells. Wound closure assay , transwell assay and adhesion a部ay were used to determine the effect of the expressìon of PI3K p85αon the invasion and migration of SW480 cells. Resultsηle healing ability , adherent ability , and invasion and migration ability of PI3K p85α­ depleted cells were significantly decreased (P 0.05). Conclusions Depletion of PI3K p85αprotein can obviously inhibit the invasion and migration of SW480 cells. And PI3K p85αmay be a new therapeutic target for human colorectal cancer. [Key words] PI3Kp85α; RNA interference; Colorectal cancer; Migration 大肠癌的发生发展涉及到癌基因的过表达和 细胞的增殖，增加大肠癌细胞对化疗药物的敏感 抑癌基因的突变、缺失等多环节、多通路的异常。导 性[町，在此基础上，我们构建了 PI3Kp85α 蛋白的慢 致大肠癌患者死亡的主要原因是出现