云芝多糖-B对人食管癌细胞Eca109转移的影响.pdfVIP

第 34 卷第3 期 中山大学学报(医学科学版) Vo 1.34 No.3 2013 年 5 月 JOURNAL OF SUN YAT-SEN UNIVERSπY(MEDICAL SCIENCES) May. 2013 云芝多糖-B 对人食管癌细胞Ecal09 转移的影响 汪道峰 l ，李小东 2 ，委宁1 (华南肿瘤学国家重点实验室//中山大学肿瘤防治中心l.ICU ， 2.胸科，广东广州 51∞ω) 摘 要:【目的】应用新型云芝多糖-B(CVPs-B)作用食管鳞癌 Ecal09 细胞，探讨其对 Ecalω 细胞转移特性的作用。[方 法] Westem blot 检测 CVPS-B 作用后，食管鳞癌 Eca109 细胞 CXCL12 蛋白表达的变化，Transwell 侵袭实验评估食管癌细胞 侵袭能力的变化，MTT 法观察食管癌细胞与 Matrigel 胶蒙古附能力的变化，为j痕法检测食管癌细胞迁移能力的变化。[结果]实 验组Eca109 细胞CXCL12 蛋白表达水平较对照组显著降低(P 0.05)0 CVPs-B 孵育后，食管癌 Ecalω 细胞蒙古附、侵袭及迁 移能力下降。[结论]CVPs-B 降低食管癌细胞 CXCL12 蛋白表达，有效抑制食管癌Ecal09 细胞的转移潜能，提示 CVPs-B 可能 可以通过 CXCL12/CXCR4 通路抑制食管鳞癌细胞转移特性。 关键词:食管癌;CVPs-B; CXCL12/CXCR4;转移 文章编号:1672-3554(2013)03-0339-06 中图分类号:R734.1 文献标志码:A EtTects of CVPs-B on Metastatic Characteristics of Human Esophageal Carcinoma Cell Line Ecal09 WANG Dao-Feng 1, Xiao-Dong Li2 , Ning lou 1 (1. Department of ICU; 2. Department of Tboracic Surgery , State Key Laboratory of Oncology in South China// Cancer Center , Sun Yat-sen University , Guangzhou , 51∞60 ， China) Abstract: [Objective] Tbe aim of this study was to explore the effects of Coriolus versicolor polysaccharide-B (CVPs-B) on the metastatic characteristics of human esophageal carcinoma cellline Ecal09 in vitro. [Methods 】 ηle expression of CXC L12 in the cell line Ecal09 was detected by Westem blot. Tbe adhesive ability of Eca109 cells to extracellular matrix matrigel was evaluated by MTT assay. The invasive ability of Eca109 cells was measured by Transwell experiment. Tbe locomotion ability of Ecal09 cells was measured by sc呻e assay. [Results 1 Compared with control g