Cloning and Characterization of a Hybridoma Secreting a 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-Specific Monoclonal Antibody and Recombinant F(ab).docVIP

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Cloning and Characterization of a Hybridoma Secreting a 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-Specific Monoclonal Antibody and Recombinant F(ab).doc

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Cloning and Characterization of a Hybridoma Secreting a 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-Specific Monoclonal Antibody and Recombinant F(ab)

Toxins 2013, 5, 568-589; doi:10.3390/toxins5030568 OPEN ACCESS toxins ISSN 2072-6651 /journal/toxins Article Cloning and Characterization of a Hybridoma Secreting a 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-Specific Monoclonal Antibody and Recombinant F(ab) Heather Wanczyk , Tolga Barker , Debra Rood , Daniel I. Zapata , Amy R. Howell 4, 1 2 1 3 4 5 6 6 Stewart K. Richardson , John Zinckgraf , Gregory P. Marusov , Michael A. Lynes and Lawrence K. Silbart 7,* 1 Department of Animal Science, University of Connecticut, Storrs, CT 06268, USA; E-Mails: heather.wanczyk@ (H.W.); debra.rood@ (D.R.) 2 National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA; E-Mail: barkertt@ 3 New York Medical College, New York, NY 10162, USA; E-Mail: dzapata84@ 4 Department of Chemistry; University of Connecticut, Storrs, CT 06268, USA; E-Mails: amy.howell@ (A.R.H.); stewart.richardson@ (S.K.R.) 5 Immucell, Portland, ME 04101, USA; E-Mail: jzinckgra@ 6 Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06268, USA; E-Mails: gregmarusov@ (G.P.M.); michael.lynes@ (M.L.) 7 Department of Allied Health Sciences, CANR, 358 Mansfield Road, University of Connecticut, Storrs, CT 06269-2101, USA * Author to whom correspondence should be addressed; E-Mail: lawrence.silbart@; Tel.: +1-860-486-0028; Fax: +1-860-486-4191. Received: 9 January 2013; in revised form: 28 February 2013 / Accepted: 5 March 2013 / Published: 19 March 2013 Abstract: Smokeless tobacco products have been associated with increased risks of oro-pharyngeal cancers, due in part to the presence of tobacco-specific nitrosamines (TSNAs) such as 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). These potent carcinogens are formed during tobacco curing and as a result of direct nitrosation reactions that occur in the oral cavity. In the current work we describe the isolation