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Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes.doc

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Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes

Virology Journal BioMedCentral Methodology Open Access Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes Myrna C Bonaldo*1, Samanta M Mello1, Gisela F Trindade1, Aymara A Rangel2, Adriana S Duarte1, Prisciliana J Oliveira1, Marcos S Freire2, Claire F Kubelka3 and Ricardo Galler2 Address: 1Funda??o Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular, de Flavivírus, Rio de Janeiro, Funda??o Oswaldo Cruz. Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ 21045-900, Brazil, 2Funda??o Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos, Rio de Janeiro, Brazil and 3Funda??o Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Imunologia Viral, Rio de Janeiro, Brazil Email: Myrna C Bonaldo* - mbonaldo@ioc.fiocruz.br; Samanta M Mello - samello@ioc.fiocruz.br; Gisela F Trindade - gfreitas@ioc.fiocruz.br; Aymara A Rangel - aymara@bio.fiocruz.br; Adriana S Duarte - adriduar@ioc.fiocruz.br; Prisciliana J Oliveira - priscilian@.br; Marcos S Freire - freire@bio.fiocruz.br; Claire F Kubelka - claire@ioc.fiocruz.br; Ricardo Galler - rgaller@bio.fiocruz.br * Corresponding author Published: 30 October 2007 Received: 22 August 2007 Accepted: 30 October 2007 Virology Journal 2007, 4:115 doi:10.1186/1743-422X-4-115 This article is available from: /content/4/1/115 ? 2007 Bonaldo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The yellow fever virus, a member of the genus Flavivirus, is an arthropod-borne pathogen causing severe disease in humans. The attenuated yellow fever 17D virus strain has been used for human vaccination for 70 years and has several characteristics that are desirable for the development of new, live att