Cowpea viruses Effect of single and mixed infections on symptomatology and virus concentration.docVIP

Virology Journal BioMedCentral Research Open Access Cowpea viruses: Effect of single and mixed infections on symptomatology and virus concentration Moni A Taiwo*1, Kehinde T Kareem1, Imade Y Nsa1 and Jackies DA Hughes2 Address: 1Dept. of Botany and Microbiology, University of Lagos, Akoka, Lagos, Nigeria and 2International Institute of Tropical Agriculture, Ibadan, Nigeria Email: Moni A Taiwo* - monitaiwo@; Kehinde T Kareem - rabkareem2@; Imade Y Nsa - imadee@; Jackies DA Hughes - j.hughes@.tw * Corresponding author Published: 27 September 2007 Received: 10 July 2007 Accepted: 27 September 2007 Virology Journal 2007, 4:95 doi:10.1186/1743-422X-4-95 This article is available from: /content/4/1/95 ? 2007 Taiwo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Natural multiple viral infections of cultivated cowpeas have been reported in Nigeria. In this study, three Nigerian commercial cowpea cultivars (Olo 11, Oloyin and White) and two lines from the IITA (IT86D- 719 and TVU 76) were mechanically inoculated with Cowpea aphid-borne mosaic virus (CABMV), Bean southern mosaic virus (SBMV) and Cowpea mottle virus (CMeV) singly, as well as in all possible combinations at 10, 20 and 30 days after planting (DAP). Samples of leaves or stems were collected at 10, 20 and 30 days after inoculation (DAI) and analyzed for relative virus concentration by Enzyme-Linked Immunosrbent Assay. All the cultivars and lines {CVS/L} were susceptible to the viruses but the commercial CVS showed more severe symptoms and had relatively higher viral concentration. In single virus infections, CABMV which induced the most severe symptoms had absorbance values (at 405 nm) of 0.11 to 0.46 while