Curbing the growth of candida-A comparative in-vitro study.docVIP

Research Article Curbing the growth of candida-A comparative in-vitro study Aruna K, *Rosaiah K Department of Conservative Dentistry Endodontics, *Department of Periodontics, People’s Dental Academy, People’s CampusBhanpur, Bhopal-462037. Abstract: Endodontics is the least forgiving branch of dentistry in terms of success or failure. Therefore, re-treatment has become a significant part of today’s endodontic practice. There is a growing evidence showing the presence of fungi especiallyCandida albicans in root canals ofobturatedteeth in which endodontic treatment has failed. It has been demonstrated that Candida species are resistant to some medications used in endodontics. The aim of this study was to examine the in- vitro susceptibilityof Candida albicans to materials used in endodontics such as mineral trioxide aggregate (MTA), Calcium hydroxide Ca(OH) /camphorated paramonochlorophenol (CMCP) /glycerine and chlorhexidine (CHX). 2 MTAand CHX are effective anti-fungal agents each with 67% anti fungal activity while Ca(OH) /CMCPshowed 2 33% anti-fungal activity. Key Words: Candida albicans; MTA; Calcium hydroxide / CMCP/ glycerin; chlorhexidine; tubedilution test. Introduction: The aim of endodontic treatment is to dextrose broth and plating onto Sabouraud’s dextrose agar plates. The cells were also observed under microscope to check their viability. One ml of Sabouraud’s dextrose broth was taken in 24 test tubes and to each, 1 ml suspension of viable C. albicans suspension was added. The tubes were divided into four equal groups. Three hundred eliminate infections from the root canal and prevention of reinfection (Nair et al, 1990). Micro-organisms isolated from primary endodontic infections are predominantly strict anaerobes. On the other hand, facultative anaerobic bacteria and yeasts prevail in therapy-resistant cases and chronic apical periodontitis as they are more resistant to antimicrobial