Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene.docVIP

下载本文档

7
0
约1.61万字
约 4页
2017-05-08 发布于上海
举报
版权申诉

Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene

Songetal.VirologyJournal2010,7:353 /content/7/1/353 SHORT REPORT OpenAccess Detectionofporcineparvovirususinga taqman-basedreal-timepcrwithprimersand probedesignedfortheNS1gene CuipingSong1,2?,ChaoZhu ,ChaofanZhang ,ShangjinCui3* 3? 3 Abstract ATaqMan-basedreal-timepolymerasechainreaction(PCR)assaywasdevisedforthedetectionofporcineparvo- virus(PPV).TwoprimersandaTaqManprobeforthenon-structuralproteinNS1geneweredesigned.Thedetec- 2 2 9 tionlimitwas1×10 DNAcopies/μL,andtheassaywaslinearintherangeof1×10 to1×10 copies/μ L.There wasnocross-reactionwithporcinecircovirus2(PCV2),porcinereproductiveandrespiratorysyndromevirus (PRRSV),pseudorabiesvirus(PRV),classicalswinefevervirus(CSFV),orJapaneseencephalitisvirus(JEV).Theassay wasspecificandreproducible.In41clinicalsamples,PPVwasdetectedin32sampleswiththereal-timePCRassay andinonly11sampleswithaconventionalPCRassay.Thereal-timeassayusingtheTaqMan-systemcantherefore bepracticallyusedforstudyingtheepidemiologyandmanagementofPPV. Introduction Incontrast,real-timePCRusingSYBRGreenandTaqMan Porcine parvovirus (PPV) isanautonomous parvovirus israpid,specific,andefficientforthelarge-scalescreening, belongingtothegenusparvovirus, subfamily Parvoviri- strainidentification,andquantificationofPPV[9]. nae,familyParvoviridae.Itisoneofthemajoretiologi- NS1, which is encoded by the NS1 gene, is a main cal agents of reproductive failure in pigs. Reproductive nonstructuralproteinofPPVandisassociatedwiththe failure caused by PPV is characterized by embryonic earlyandlatetranscriptionofthevirus.Giventhatinac- andfoetaldeath,mummification,stillbirth, anddelayed tivated virus used in current vaccines have only little return to oestrus [1]. Inaddition, PPV has been impli- NS1proteinwhichcouldnotproduceantibody,thepre- cated as the causative agent of diarrhea, skin disease, senceorabsenceofantibodyagainstNS1proteincould andarthritis inswine [2]. PPVhas been reported from beusedinanNS1-baseddiagnostickitfordetermining m