Effect of Azadirachta indica A. Juss (Meliaceae) Seed Oil and Extract Against Culex quinquefasciatus Say (Diptera Culicidae) Larval Susceptibility of Indian Subcontinent.docVIP

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Effect of Azadirachta indica A. Juss (Meliaceae) Seed Oil and Extract Against Culex quinquefasciatus Say (Diptera Culicidae) Larval Susceptibility of Indian Subcontinent.doc

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Effect of Azadirachta indica A. Juss (Meliaceae) Seed Oil and Extract Against Culex quinquefasciatus Say (Diptera Culicidae) Larval Susceptibility of Indian Subcontinent

Mandal. Effect of Azadirachta indica A. Juss Seed Oil and Extract Against Culex quinquefasciatus Say Macedonian Journal of Medical Sciences. 2011 Mar 15; 4(1):5-11. doi:10.3889/MJMS.1857-5773.2011.0127 Basic Science OPEN?ACCESS Effect of Azadirachta indica A. Juss (Meliaceae) Seed Oil and Extract Against Culex quinquefasciatus Say (Diptera: Culicidae) Larval Susceptibility of Indian Subcontinent Shyamapada Mandal Department of Microbiology, Bacteriology and Serology Unit, Calcutta School of Tropical Medicine, C. R. Avenue, Kolkata-700 073, India Abstract Citation: Mandal S. Effect of Azadirachta indica A. Juss (Meliaceae) Seed Oil and Extract Against Culex quinquefasciatus Say (Diptera: Culicidae) LarvalSusceptibilityofIndianSubcontinent.Maced J Med Sci. 2011 Mar 15; 4(1):5-11. doi.10.3889/ MJMS.1957-5773.2011.0127. Background. Development of resistance to chemical pesticides among the mosquito vectors leads researchers to investigate the potential mosquito control efficacy of various plant extracts. Aim. To assess the larvicidal activity of neem (Azadirachta indica A. Juss; Meliaceae) seed extract and oil against Culex quinquefasciatus Say (Family: Culicidae). Key words: Azadirachta indica ; quinquefasciatus larva; Probit analysis; LC50; LT50 Culex Materials and Methods. In order to determine the median lethal concentration (LC ), following probit analysis the fourth instar larvae of C. quinquefasciatus were exposed to A. indica seed extract and oil in different concentrations ranging from 2 to 64 μg/ml, and larval mortality was observed for each 50 . Correspondence:Dr.ShyamapadaMandal. De- partment Narkeldanga, samtropmed@ of Zoology, Kolkata-700 054, India. E-mail: Gurudas College, concentrationafter24hoursoftreatment.Themedianlethaltime(LT )valuesforasingleconcentration 50 (2 x LC ) of oil and extract were determined based upon probit analysis. 50 Received: 04-Apr-2010; Revised: 01-Aug-2010; Accepted:03-Sep-2010;Onlinefirst:16-Jan-2