Antigenic Proteins Involved in Occupational Rhinitis and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon).docVIP

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Antigenic Proteins Involved in Occupational Rhinitis and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon).doc

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Antigenic Proteins Involved in Occupational Rhinitis and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon)

AntigenicProteinsInvolvedinOccupationalRhinitisand AsthmaCausedbyObecheWood(Triplochiton Scleroxylon) AnaAranda1,PalomaCampo2*,ArantxaPalacin3,InmaculadaDon?a2,CristinaGomez-Casado3, LuisaGalindo2,AraceliD?′az-Perales3,MiguelBlanca2 1AllergyResearchLaboratory,CarlosHayaHospital,Ma′laga,Spain,2UnidaddeGestio′nCl?′nicaAllergy,CarlosHayaHospital,Ma′laga,Spain,3BiotechnologyDepartment, CenterforPlantBiotechnologyandGenomics,PozuelodeAlarcon,Madrid,Spain Abstract Background:ObechewooddustisaknowncauseofoccupationalasthmawhereanIgE-mediatedmechanismhasbeen demonstrated. Objective:Tocharacterizetheallergenicprofileofobechewooddustandevaluatethereactivityoftheproteinsbyinvitro, exvivoandinvivoassaysincarpenterswithconfirmedrhinitisand/orasthma Materials and methods: An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12kDa)andsequencedbyN-terminalidentity.SpecificIgEandIgG,basophilactivationtestsandskinpricktests(SPTs)were performedwithwholeextractandpurifiedproteins.CCDbindingwasanalyzedbyELISAinhibitionstudies. Results:Sixty-twosubjectsparticipated:12withconfirmedoccupationalasthma/rhinitis(ORA+),40asymptomaticexposed (ORA2),and10controls.Oftheconfirmedsubjects,83%hadapositiveSPTtoobeche.Therewasa100%recognitionby ELISAinsymptomaticsubjectsvs.30%and10%inasymptomaticexposedsubjectsandcontrolsrespectively(p,0.05).Two newproteinswerepurified,a24kDaproteinidentifiedasaputativethaumatin-likeproteinanda12kDagamma-expansin. Bothshowedallergenicactivityinvitro,withtheputativethaumatinbeingthemostactive,with92%recognitionbyELISA and100%bybasophilactivationtestinORA+subjects.Cross-reactivityduetoCCDwasruledoutin82%ofcases. Conclusions: Two proteins of obeche wood were identified andwere recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivitywithotherplantallergens. Citation: Aranda A, Campo P, Palacin A, Don?a I, Gomez-Casado C