Association of Lipidome Remodeling in the Adipocyte Membrane with Acquired Obesity in Humans.docVIP

AssociationofLipidomeRemodelingintheAdipocyte MembranewithAcquiredObesityinHumans KirsiH.Pietila¨inen1,2,3.,TomaszRo′g4.,TuulikkiSeppa¨nen-Laakso5.,SamVirtue6. ,Peddinti Gopalacharyulu5,JingTang5,SergioRodriguez-Cuenca6,ArkadiuszMaciejewski4,7 ,Jussi Naukkarinen8,9,Anna-LiisaRuskeepa¨a¨5,PerttuS.Niemela¨5,LaxmanYetukuri5,ChongYewTan6 ,Vidya Velagapudi5,SandraCastillo5,HeliNygren5,TuuliaHyo¨tyla¨inen5,AilaRissanen1,JaakkoKaprio2,3,9, HanneleYki-Ja¨rvinen10,IlpoVattulainen4,11,12,AntonioVidal-Puig6,MatejOresˇicˇ3,5* 1Department of Medicine, Division of Internal Medicine, and Department of Psychiatry, Obesity Research Unit, Helsinki University Central Hospital, Helsinki, Finland, 2Department of Public Health, Hjelt Institute, University of Helsinki, Helsinki, Finland, 3Institute for Molecular Medicine Finland, Helsinki, Finland, 4Department of Physics, Tampere University of Technology, Tampere, Finland, 5VTT Technical Research Centre of Finland, Espoo, Finland, 6Institute of Metabolic Science, Metabolic ResearchLaboratories,UniversityofCambridge,Addenbrooke’sHospital,Cambridge,UnitedKingdom,7DepartmentofComputationalBiophysicsandBioinformatics, JagiellonianUniversity,Krako′w,Poland,8DepartmentofMedicalGenetics,UniversityofHelsinki,Helsinki,Finland,9DepartmentofMentalHealthandSubstanceAbuse Services, National Institute for Health and Welfare, Helsinki, Finland, 10Division of Diabetes, Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland, 11Department of Applied Physics, School of Science and Technology, Aalto University, Espoo, Finland, 12MEMPHYS—Center for Biomembrane Physics, UniversityofSouthernDenmark,Odense,Denmark Abstract Identification of early mechanisms that may lead from obesity towards complications such as metabolic syndrome is of great interest. Here we performed lipidomic analyses of adipose tissue in twin pairs discordant for obesity but still metabolically compensated. In parallel we studied more evolved states of obesity by