Association of Neisseria gonorrhoeae OpaCEA with Dendritic Cells Suppresses Their Ability to Elicit an HIV-1-Specific T Cell Memory Response.docVIP

下载本文档

2
0
约7.02万字
约 13页
2017-05-07 发布于上海
举报
版权申诉

Association of Neisseria gonorrhoeae OpaCEA with Dendritic Cells Suppresses Their Ability to Elicit an HIV-1-Specific T Cell Memory Response.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Association of Neisseria gonorrhoeae OpaCEA with Dendritic Cells Suppresses Their Ability to Elicit an HIV-1-Specific T Cell Memory Response

AssociationofNeisseriagonorrhoeaeOpaCEA with DendriticCellsSuppressesTheirAbilitytoElicitanHIV-1- SpecificTCellMemoryResponse QiguiYu1*,EdithM.C.Chow2,ShannonE.McCaw2,NingjieHu1,DanielByrd1,TohtiAmet1,SishunHu1, MarioA.Ostrowski,ScottD.Gray-Owen2* 1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America, 2Department of Molecular Genetics,and3ClinicalSciencesDivision,UniversityofToronto,Toronto,Ontario,Canada Abstract Infection with Neisseria gonorrhoeae (N. gonorrhoeae) can trigger an intense local inflammatory response at the site of infection,yetthereislittlespecificimmuneresponseordevelopmentofimmunememory.Gonococcalsurfaceepitopesare knowntoundergoantigenicvariation;however,thisisunlikelytoexplaintheweakimmuneresponsetoinfectionsince individualscanbere-infectedbythesameserotype.Previousstudieshavedemonstratedthatthecolonyopacity-associated (Opa)proteinsontheN.gonorrhoeaesurfacecanbindhumancarcinoembryonicantigen-relatedcellularadhesionmolecule + 1 (CEACAM1) on CD4 T cells to suppress T cell activation and proliferation. Interesting in this regard, N. gonorrhoeae infectionisassociatedwithimpairedHIV-1(humanimmunodeficiencyvirustype1)-specificcytotoxicT-lymphocyte(CTL) responsesandwithtransientincreasesinplasmaviremiainHIV-1-infectedpatients,suggestingthatN.gonorrhoeaemay alsosubvertimmuneresponsestoco-pathogens.Sincedendriticcells(DCs)areprofessionalantigenpresentingcells(APCs) thatplayakeyroleintheinductionofanadaptiveimmuneresponse,weinvestigatedtheeffectsofN.gonorrhoeaeOpa proteinsonhumanDCactivationandfunction.WhilemorphologicalchangesreminiscentofDCmaturationwereevident uponN.gonorrhoeaeinfection,weobservedamarkeddownregulationofDCmaturationmarkerCD83whenthegonococci expressing CEACAM1-specific OpaCEA, but not other Opa variants. Consistent with a gonococcal-induced defect in maturation,OpaCEAbindingtoCEACAM1reducedtheDCs’capacitytostimulateanallogeneicTcellproliferativeresponse. M