Association Study of rs13241278 and rs2693657 Polymorphisms of PTPRZ1 Gene with Multiple Sclerosis in Iranian Population.docVIP

Original Article Association Study of rand rs2693657 Polymorphisms of PTPRZ1 Gene with Multiple Sclerosis in Iranian Population Zahra Bahadori, M.Sc. 1 , Mehrdad Behmanesh, Ph.D. *, Mohammad-Ali Sahraian, M.D. , 1 2 Moones Heidari, M.Sc. 1 1. Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran 2. Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran * Corresponding Address: P.O.Box: 14115-154, Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Email: behmanesh@modares.ac.ir Received: 23/Feb/2010, Accepted: 13/Jun/2010 Abstract Objective: Human multiple sclerosis (MS) is a complex disease and demyelinated lesions in central nervous system (CNS) are the pathologic hallmark of MS. Remyelination occurs in many MS lesions but becomes increasingly incomplete/inadequate. Protein tyrosine phosphatase,receptor-type z polypeptid1 (PTPRZ1) has been implicated in adult cell re- newal, repair of the nervous system, oligodendrocyte development and so in Remyelina- tion. We investigated possible association of multiple sclerosis with polymorphism of two SNPs (rand rs2693657) located in PTPRZ1 gene. Materials and Methods: Peripheral blood was collected from 140 subjects with MS and 165 healthy controls and DNA was extracted. For genotyping of rand r PCR-RFLP and mismatch PCR-RFLP techniques were used, respectively. Association of SNPrand SNPr with multiple sclerosis was exam- ined by using the Chi-square test and the frequency differences of alleles and genotypes between two groups were compared. A conventional p-value of ≤ 0.05 was considered significant. Results: Statistical analyses on two studied polymorphisms showed that both case and 2 control group were in Hardy-Weinberg equilibrium. By