Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems 英文论文文献.docVIP
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Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems 英文论文文献
Lauteretal.BMCDevelopmentalBiology2011,11:43
/1471-213X/11/43
METHODOLOGY ARTICLE
OpenAccess
Two-colorfluorescentinsituhybridizationinthe
embryoniczebrafishbrainusingdifferential
detectionsystems
GilbertLauter,IrisS?llandGiselbertHauptmann*
Abstract
Background:Whole-mountinsituhybridization(WISH)isextensivelyusedtocharacterizegeneexpressionpatterns
indevelopingandadultbrainandothertissues.Toobtainanideawhetheranovelgenemightbeinvolvedin
specificationofadistinctbrainsubdivision,nucleusorneuronallineage,itisoftenusefultocorrelateitsexpression
withthatofaknownregionalorneuronalmarkergene.Two-c
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