三种常用方法保存临床常见菌种的比较.pdfVIP

第 24 卷第3 期 甘肃科学学报 Vol. 24 No.3 2012 年 9 月 Journal of Gansu Scíences Sep.2012 三种常用方法保存临床常见菌种的比较 彭俊华，薛荣利，张缓缓，朱晓红，李泽慧 〈兰州军区兰州总医院检验科，甘肃兰州 730050) 摘 要z 比较3 种保存临床常见菌种常用方法.临床分离非苛养菌 550 株分JlJ Jij半固体.脂法、细 菌湿种牛奶冻存法和纸片冻存法进行保存，比较保存 1 个月、12 个月、36 个月后 3 种方法的菌种存 活率.半固体珠脂法、细菌湿种牛奶冻存法和纸片冻存法保存 1 个月时的菌袜子均存活率分别为 98.6% 、 100%和 100% .36 个月时的菌株平均存活率分别为 45.1% 、 90.6%和 86.1%-4存法在长 时间保存菌株时效果明显优于半团体琼脂法.3 种方法对化良性链球菌的保存效果不佳.结果表明， 3 种方法均能很好地保存临床常见菌，方法简便易行，各实事金室可依具体要求选用. 关键词z 菌种保存p 细菌存活率F 冻存法 中固分类号: R446.5 文献标志码: A 文章编号:1004-0366(2012)03-0088-03 Comparison of Three Methods for Common ClinicaI Bacterial Preservation PENG Jun-hua.XUE Rong-li.ZHANG Yuan-yuan.ZHU Xiao-hong.LI Ze-hui (Department 01 Clinical Laboratory •Lanzhou General Hospital •Lanzhou Command .PLA. Lanzhou 130050.China) Abstract: Three ordinary methods of preverving common clinical bacteria were compared. The semi-solid method (SSM) .the milk freezing method (MFM) .and the paper freezing method (PFM) were used to pre- serve 550 strains of non-fastidious bacteria from clinical isolates. Their bacterial survival rates (BSR) were compared respectively after bacterial preservation for 1.12.36 months. The resùlts showed that the BSR of SSM.MFM.PFM were 98.6% .100% and 100% after preserving for 1 month.respectively. The BSR of SSM.MFM.PFM were 45. 1% .90.6% and 86. 1% after preserving for 36 months.respectively. The stor- age effect of freezing methods was better than that of SSM after long time preservation. But for three meth- o巾.the preservation of streptococcus was not good. As a result. the three methods are good for the com- mon clinical isolates; they are sim