白纹伊蚊及致倦库蚊对蝙蝠乙型脑炎病毒分离株的易感性分析.PDFVIP

2012;32(4) 南方医科大学学报（J South Med Univ ） ·515 · 基础研究 白纹伊蚊及致倦库蚊对蝙蝠乙型脑炎病毒分离株的易感性分析 1 1 1 1 2 1 刘 姗 ，张琼花 ，周军华 ，俞守义 ，郑学礼 ，陈 清 1 2 南方医科大学公共卫生与热带医学学院 流行病学系，病原生物学系，广东 广州 510515 摘要：目的 了解蝙蝠乙脑病毒分离株GD1和HN2 株对白纹伊蚊和致倦库蚊的易感性。方法通过病毒液饲喂法使白纹伊蚊和 致倦库蚊经口感染蝙蝠乙脑病毒GD1株和HN2 株，采用TaqMan real-time RT-PCR 方法，检测GD1株和HN2 株感染白纹伊蚊及 致倦库蚊后第4、6、8、10、12、14、16、18、20 天病毒存在情况与病毒载量的动态变化。结果两株蝙蝠乙脑病毒在感染白纹伊蚊、 致倦库蚊后4~20 d 内均能被检测到。Ct值的动态变化规律显示白纹伊蚊和致倦库蚊均能感染乙脑病毒GD1株和HN2 株，但致 倦库蚊的易感性高于白纹伊蚊；两株毒株的毒力可能不同，HN2 株毒力大于GD1株。结论白纹伊蚊和致倦库蚊均能感染蝙蝠 乙脑病毒分离株。 关键词：白纹伊蚊；致倦库蚊；蝙蝠；乙型脑炎病毒；感染 中图分类号：R384.1; R373.31 文献标志码：A 文章编号：1673-4254（2012）04-0515-04 doi: 10.3969/j.issn. 1673-4254.2012.04.016 /kcms/detail/44.1627.R1744.011.html Susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to infection with bat Japanese encephalitis virus isolates 1 1 1 1 2 1 LIU Shan , ZHANG Qionghua , ZHOU Junhua , YU Shouyi , ZHENG Xueli , CHEN Qing 1Department of Epidemiology, 2Department of Etiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China Abstract: Objective To evaluate the susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to oral infection with bat Japanese encephalitis virus isolates (GD1 and HN2 strains). Methods Aedes albopictus and Culex pipiens quinquefasciatus were infected orally by GD1 and HN2 strains of bat Japanese encephalitis virus. TaqMan real-time PCR was used to detect the virus and monitor the changes in the viral loads in Aedes albopictus and Culex pipiens quinquefasciatus at a 2-day interval, starting from 4 days till 20 days after the infection. Results The infected Ae