平菇栽培菌株与野生菌株杂交子农艺性状的遗传分化.docVIP

Hans Journal of Agricultural Sciences农业科学, 2011, 1, 7-13 /10.12677/hjas.2011.12002 Published Online December 2011 (/journal/hjas) Genetic Differentiation of Hybrids from Cultivated Strain and Wild Strain in Oyster Mushroom Pleurotus ostreatus* Yuxia Zhang, Zhangyi Xu, Yingli Cai, Yinbing Bian # Institute of Applied Mycology, Huazhong Agricultural University, Wuhan Email: bianyb.123@163.com Received: Sep. 15th, 2011; revised: Oct. 20th, 2011; accepted: Oct. 24th, 2011. Abstract: Cultivated strain HP and wild strain ZJJ of Pleurotus ostreatus were used in this study. 38 mono- karyons from strain HP and 55 monokaryons from strain ZJJ were obtained respectively by single basi- diospore isolation. 10 monokaryons from each parental strain were selected randomly for hybridization and 14 dikaryons were obtained. According to the different antagonist reaction between hybrid and parents, the 14 hybrids were divided into 4 types. The mycelia growth rate on different media showed dominant differ- rentiation. The result of cultivation test showed that one hybrid could not fruit and yield of the others were lower than that of the cultivated strain HP, while the yields of 5 hybrids were higher than that of the wild strain ZJJ. Among the hybrids, most pilei showed similar color with that of parent HP (white), 3 hybrids formed fruitbodies without pilei. The analysis of hybrids agronomic charateristics showed that the coefficient of variation (CV) of yield was the largest (21.9%), the others in the order were primordial initiation days (25.4%), stipe length (21.8%),full colonization time (21.0%),average weight of single fruitbody (16.2%), pi- leus thickness (14.5%), pileus diameter (13.5%) and stipe diameter (9.4%). Keywords: Hybridization; Trait; Genetic Differentiation 平菇栽培菌株与野生菌株杂交子农艺性状的遗传分化* 张玉霞，徐章逸，蔡英丽，边银丙# 华中农业大学应用真菌研究所，武汉 Email: bianyb.123@163.com 收稿日期：2011年 9月 15日；修回日期：2011年 10月 20日；录用日期：2011年