新生大鼠神经干细胞的体外培养与冷冻复苏.docVIP

Hans Journal of Biomedicine 生物医学, 2011, 1, 25-33 /10.12677/hjbm.2011.12005 Published Online October 2011 (/journal/hjbm/) Cryopreservation and Culture of Neural Stem Cells Isolated from Postnatal Rat Jing Zhang , Xianjiang Kang , Chen Zhou , Ping Zhang , Shumei Mu , Han Zhang 1 1 1 2 1 1 1 College of Life-Science, Hebei University, Baoding 2 Affiliated Hospital of Hebei University, Baoding Email: bingyujing1014@163.com; xjkang@ Received: Sep. 1st, 2011; revised: Sep. 17th, 2011; accepted: Sep. 19th, 2011. Abstract: Objective: to isolate and culture neural stem cells from postnatal rat and to observe the viability and biological property of these cells after cryopreservation. Methods: the neural stem cells isolated from hippocampus of the postnatal rat were cultured in culture solution without blood serum. After amplification, the cells were cultured in different concentration of freeze-stored liquid which contained 0, 5%, 10%, 15%, 20% DMSO and fetal calf serum, then stored in refrigerator of 80 centigrade below zero one week, two weeks and one month respectively. Through resuscitation training and testing, then we detected the recovery rate of the NSCs. Results: after cryopreservation and culture of neural stem cells isolated from postnatal rat, most of them were growth well and formed into the new cell clones, then the recovery rate of the cells were detected with Trypan Blue. There was significant difference in 10% DMSO and it has the highest recovery rate, which is 54.00 ± 1.73, 59.00 ± 1.16, 58.00 ± 2.08. Conclusion: the neural stem cells derived from postnatal rat were able to culture, cryopreservation and resuscitation, which did not change their biological properties. Keywords: Neural Stem Cells; Postnatal Rat; Culture in Vitro; Cryopreservation; Resuscit