金霉素发酵罐补糖速率优化调节方法研究 .pdfVIP

学兔兔 第35卷 第2期 仪 器 仪 表 学 报 Vo1．35 No．2 2014年2月 Chinese Journal of Scientific Instrument Feb．2014 金霉素发酵罐补糖速率优化调节方法研究 杨建文，陈祥光，金怀平，吴 磊 (北京理T大学化T与环境学院 北京 100081) 摘 要：目前，金霉素发酵生产过程的补糖速率控制由人T操作完成，导致发酵过程的总糖浓度不稳定而影响发酵效率。为 此，提出了一种基于培养基总糖浓度软测量的补糖速率优化调节新方法、 通过发酵罐可测变量选取、数据的模糊化处理、递归 模糊神经网络构建和网络滚动训练。建 总糖浓度在线软测量模型。并且在总糖浓度软测量的基础J二，将发酵罐尾气巾CO 含量的变化过程划分为4个控制阶段，依此对补糖速率实现优化调节。现场的试验结果表明该方法的实施有利于菌体生长和 金霉素合成，提高了发酵效率并节约了生产成本。 关键词：金霉素发酵；补糖速率；优化调节；软测量 中图分类号：TP273 文献标识码：A 国家标准学科分类代码：120．30 Study on the industrial chl0rtetracycline fermentor glucose feed rate adjustment method based on soft sensor Yang Jianwen，Chen Xiangguang，Jin Hnaiping，Wu Lei (School of Chemical Engineering and Environment，Beijing Institute of Technology，Beijing 100081，China) Abstract：The glucose feed rate of Chlortetracycline(CTC)fermentation process is currently controlled manually in bi— ochemical plant，which results in the instability of total sugar concentration and affects the fermentation efficiency of the fermentation process．Therefore，we present a new optimal adjustment method for the glucose feed rate based on the soft sensor of the total sugar concentration of fermentation broth．We establish an online soft sensor model for the total sugar concentration by means of selecting the measurable variables，blurring the data，constructing the recurrent fuzzy neural network and rolling training the network．On the base of the soft sensor for total sugar concentration，the changing process of the CO2 content in the fermentor exhaust gas is divided into four control phases，with wh