尿液内源性类固醇.pdf

6 1 Vol. 6 No. 1 2015 1 Journal of Food Safety and Quality Jan. , 2015 刘慧燕* (, 528116) 摘 要: 目的 , (gas chromatography-mass spectrometry, GC-MS)方法 , , β-, SIM 结果 0.2~0.5 ng/mL, 93.64%~116.74%, (relative standard deviation, RSD)6.59%结论 关键词: -; ; Solid phase extraction and determination of endogenous anabolic steroids in urine by gas chromatography-mass spectrometry * LIU Hui-Yan (Guangdong Vocational College of Environmental Protection Engineering, Foshan 528116, China) ABSTRACT: Objective To develop a method of gas chromatography-mass spectrometry (GC-MS) for the simultaneous identification and quantification of endogenous anabolic steroids in urine of athletes for assurance of their food and drug safety. Methods The thawed iced urine sample was centrifuged, and the liquid supernatant was extracted using reverse phase C18 column. The methanol eluant was collected, hydrolized by β-glucuronidase, derivatized with a derivatization reagent and detected using GC-MS in SIM mode. Results The limits of detection for endogenous anabolic steroids were in the range of 0.2~0.5 ng/mL, the average recoveries were 93.64%~116.74% with relative standard deviation (RSD) less than 6.59%. Conclusi