手持式ATP生物荧光检测仪研制_周爱玉.pdf

第2 1 卷　第4 期 传 感 技 术 学 报 V ol .21　No.4 2008 年4 月 CHINESE JOURNAL OF SENSORS AND ACT UATORS Apr.2008 ＊ Development of Handheld ATP Detecting System Based on Bioluminescence 1,2 1,2 1,2 1,2 1,2 1,2 ＊ Z HOU Ai-y u ,L UOJ in-p ing ,YUE Wei-wei ,HE Bao-shan ,YA N G Qing -de , CA I X in-xia 1.tateK ey Laboratory of Transducer Technology , Institute of E lectronics, Chinese Academy of ciences, Beij ing 100080, China; 2.Graduate chool of Chinese Academy of ciences, Beij ing 100080, China Abstract:A handheld Adenosine Triphosphate (A TP)detecting system based on bioluminescence w as de- scribed.In the system, a small photomultiplier tube (PMT )module w as used to measure the weak biolu- minescence released from ATP reaction.The system w as used to detect A TP in the range of 10-1 ～10-9 mol/L.The results w ere compared to that of fluorescence spectrophotometer F4 00 w hich show ed the low est A TP concentration the system could detected w as 10-14 mol/L w hich w as 1/100 of that F4 00 could do.In 10s time, the response signals correlated w ell to the ATP concentration in the range of 10-14 ～ 10-9 mol/L w ith the correlation coefficient of 0.9902. Key words:adenosine triphosphate;bioluminescence;handheld detecting system;photomultiplier tube EEACC:320T AT P ＊ 1, 2 1, 2 1, 2 1, 2 1, 2 1, 2 ＊ 周爱玉 , 罗金平 ,岳伟伟 ,何保山 ,杨庆德 ,蔡新霞 (1., 100080;2., 100080) 　:(ATP), , ATP 。 PM T , , A TP 。 10-1 ～ 10-9 mol/L ATP , F4 00 , , , ATP 10-14 mol/L, F4 00 1/100。 10 s , 10-14 ～ 10-9 mol/L , ATP , 0.9902。 ;;; : :TJ81.0.36　　 :A　　:1004-1699 (2008)04-0543-04 　　(Adenosine Triphosphate, A TP) 1　 , 。 AT P, 、