下一代测序技术在表观遗传学研究中的重要应用及进展_沈圣.pdfVIP

Hereditas (Beijing) 2014 3 , 36(3): 256275 , , , 79905 (Next generation sequencing, NGS), NGS , NGS , (Whole genome bisulfite sequencing, WGBS)(Reduced representation bisulfite sequencing, RRBS)DNA (Methylated DNA immunoprecipitationsequencing, MeDIP-seq) (Chromatin immunoprecipitation-sequencing, ChIP-seq) Tet (Tet-assisted bisulfite sequencing, TAB-seq)(Chromosome conformation capture se- quencing, 3C-seq) Dnase -seq/MNase-seq/FAIRE-seq RNA (RNA sequencing, RNA-seq) , , NGS , NGS , NGS ; ; DNA ; ; The application of next generation sequencing on epigenetic study Sheng Shen, Yanchun Qu, Jun Zhang COE for Neuroscience, Texas Tech University Health Sciences Center, El Paso, Texas 79905, USA Abstract: The application of next generation sequencing (NGS) technique has a great impact on epigenetic studies. Coupled with NGS, a number of sequencing-based methodologies have been developed and applied in epigenetic studies, such as Whole Genome Bisulfite Sequencing (WGBS), Reduced Representation Bisulfite Sequencing (RRBS), Methylated DNA Immunoprecipitation Sequencing (MeDIP-seq), Chromatin Immunoprecipitation-Sequencing (ChIP-seq), TAB-seq (Tet-assisted Bisulfite Sequencing), Chromosome Conformation Capture Sequencing (3C-seq) and various of 3C-seq deriv- atives, DNase -seq/MNase-seq/FAIRE-seqand RNA Sequencing (RNA-seq). These new