Analysis of local and systemic inflammatory responses induced by polymicrobial peritonitis in mice英文文献资料.docVIP

Research Paper Mediators of Inflammation, 10, 237–243 (2001) B ACKGROUND : Abdominal sepsis induces a local pro- Analysis of local and systemic inflammatory responses induced by polymicrobial peritonitis in mice duction of proinflammatory mediators that may trigger both septic shock and organ-system dysfunction. Aims: The present study analyzed exudation, cell migration, and CD11a and CD18 subset cells of both local and systemic responses induced by fecal perito- nitis in mice. Ta?nia Silvia Fro¨ de1,CA, Silvia Ines Alejandra Cordoba Methods: Animals were anesthetized and, after per- forming a midline incision in the abdomen, the cecum was ligated and punctured twice with a needle. Sham-operated animals were included. Some groups were previously treated with Evans blue dye (intra- venously)tofurtherevaluatetheamountoftissueand abdominalcavityleakages. Pires Ferreira and Yara Santos Medeiros 2 3 1 Department of Clinical Analysis, Centre of Healthy Laboratory of Santa Luzia and Department of Pharmacology, Centre of Biological Sciences, 3 2 Results: Fecalperitonitis triggered a local inflamma- tory reaction with an increased number of leukocytes Sciences, CCS, Universidade Federal de Santa Catarina, Campus Universita′rio – Trindade, 88040–970 Floriano′polis, SC, Brazil and exudation between 6 and 48h (p 0.01). Although CD11a/CD18-positive cells in the abdomen peaked after 24h, a significant decrease of them was detected after 48h (p 0.05). At the studied period of time (6D48h), different degrees of exudation in several organs occurred, whereas a significant late recruitment (24h) of CD11a/CD18 cells into the lungs was observed. CA Corresponding Author Fax: (+55) 48 2 44-09 36 E-mail: saleh@ccs.ufsc.br Conclusions: In this model, cell migration and exuda- tion at the site of injury occurred in parallel. How- ever, in the lungs, the recruitment of leukocytes that express CD11a/CD18