Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells英文文献资料.docVIP

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Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells英文文献资料.doc

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Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells英文文献资料

HindawiPublishingCorporation MediatorsofIn?ammation Volume2012,ArticleID265714,6pages doi:10.1155/2012/265714 ResearchArticle AzithromycinInhibitsMucusHypersecretionfrom AirwayEpithelialCells TakeshiShimizuandShinoShimizu DepartmentofOtorhinolaryngology,ShigaUniversityofMedicalScience,Seta,Tsukinowa,Otsu,Shiga520-2192,Japan CorrespondenceshouldbeaddressedtoTakeshiShimizu,Shimizu@belle.shiga-med.ac.jp Received16January2012;Revised27February2012;Accepted2March2012 AcademicEditor:KazuhitoAsano Copyright?2012T.ShimizuandS.Shimizu.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. To examine the in vivo e?ects of the 15-member macrolide, azithromycin (AZM), on mucus hypersecretion, we induced hypertrophicandmetaplasticchangesofgobletcellsinratnasalepitheliumbyintranasalinstillationofovalbumin(OVA)inOVA- sensitizedrats,orbyintranasallipopolysaccharides(LPS)instillation.OraladministrationofAZM(5–10mg/kg)orclarithromycin (CAM, 5–10mg/kg) signi?cantly inhibited OVA- and LPS-induced mucus production, whereas josamycin (JM) or ampicillin (ABPC)showednoe?ect.Invitroe?ectsofAZMonairwayepithelialcellswereexaminedusingNCI-H292cellsandhumannasal epithelialcellsculturedinair-liquidinterface.Mucussecretionwasevaluatedbyenzyme-linkedimmunosorbentassayusingan anti-MUC5ACmonoclonalantibody.AZMorCAMsigni?cantlyinhibitedtumornecrosisfactor-α(TNF-α)(20ng/mL)-induced MUC5ACsecretionfromNCI-H292cellsat10?6 –10 ?7M,whereasJMorABPCshowednoe?ect.AZMsigni?cantlyinhibited TNF-α(20ng/mL)-inducedMUC5ACsecretionfromhumannasalepithelialcellsat10?4M.MUC5ACmRNAexpressionwas alsosigni?cantlyinhibited.Theseresultsindicatethatthe15-membermacrolide,AZM,exertsdirectinhibitorye?ectsonmucus secretion from airway epithelial cells and that it may be useful for the treatment of mucus hypersecretion caused by allergic in?ammationandLPSstimulation. 1.Introduction mucus producti