Building reliable genetic maps different mapping strategies may result in different maps英文文献资料.docVIP

Vol.2, No.6, 576-589 (2010) Natural Science /10.4236/ns.2010.26073 Building reliable genetic maps: different mapping strategies may result in different maps Yefim Ronin, David Mester, Dina Minkov, Abraham Korol* Institute of Evolution and Department of Evolutionary and Environmental Biology University of Haifa, Mount Carmel, Haifa, Israel; *Corresponding Author: korol@research.haifa.ac.il Received 24 November 2009; revised 4 January 2010; accepted 6 April 2010. ABSTRACT objective of our study is to develop efficient methodol- ogy for building multilocus genetic maps, providing the control of the quality of maps by detecting and removing the sources of map instability. Two major problems should be addressed in multilocus genetic mapping: 1) markers that belong to non-homologous chromosomes should not be assigned to the same linkage group; and 2) markers from the same chromosome should be placed on the genetic map in the same order as the corresponding DNA sequences that reside in the chromosome. In situations with significant deviations of the recom- bination rates between non-synthetic markers from the expected level (50%), the problem of correct clustering cannot be solved by an arbitrary choice of a certain (constant) threshold value of recombination or LOD, albeit this is exactly how this problem is treated in many mapping packages [1,2]. Indeed, in experiments with the foregoing characteristics, the recombination values be- tween groups of markers from different chromosomes may sometimes be smaller than the values between ad- jacent markers within a chromosome. This phenomenon, referred to as “quasi-linkage” (or “pseudo-linkage”) can result from a combination of statistical and biological reasons and scoring errors. The statistical reasonsof pseudo-linkage are mainly caused by the sample size and number of chromosomes (n) in the genome: increased n is associated with hig