Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat英文文献资料.docVIP

American Journal of Plant Sciences, 2011, 2, 467-475 467 doi:10.4236/ajps.2011.23055 Published Online September 2011 (http://www.SciRP.org/journal/ajps) Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat Ahmed Hassanein , Ibrahim A. Ibraheim , Abel-Naser Galal , Jehan Salem 1 2 1 1 1 Central Laboratory of Genetic Engineering, Faculty of Science, Sohag University, Sohag, Egypt; 2 Genetic Engineering and Biotech- nical Laboratory, Sadat City, Monofia University, Monofia, Egypt. Email: {hassaneinam, ahmosman2000}@ Received April 26 , 2011; revised June 10 , 2011; accepted June 23 , 2011. th th rd ABSTRACT Banana shoot tips extracted from in vitro grown plant materials were encapsulated in different types of alginate beads. The results obtained after conservation for one month at 4?C indicated that single layered synseeds (where the encap- sulated shoot tip was covered by one layer of the artificial endosperm) containing activated charcoal were the best con- struction in stimulating conversion of synseeds, where they expressed the highest; conversion frequency, number of shoots/synseed, length of shoot and fresh weight/shoot cluster. These parameters decreased when the shoot tips were encapsulated within two layers of endosperm, except the ex vitro conversion. Conversion of banana synseeds was in- fluenced by explant orientation inside the alginate beads, the highest conversion frequency was obtained when the shoot tip was directed upward inside the synthetic endosperm. Synthesis of seed coats around the artificial endosperm im- proved the conversion frequency, number of shoots/synseed and shoot length. Keywords: Artificial Seeds, Banana, Conservation, Synsee