A Barcode Screen for Epigenetic Regulators Reveals a Role for the NuB4HAT-B Histone Acetyltransferase Complex in Histone Turnover 英文参考文献.docVIP

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A Barcode Screen for Epigenetic Regulators Reveals a Role for the NuB4HAT-B Histone Acetyltransferase Complex in Histone Turnover 英文参考文献.doc

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A Barcode Screen for Epigenetic Regulators Reveals a Role for the NuB4HAT-B Histone Acetyltransferase Complex in Histone Turnover 英文参考文献

ABarcodeScreenforEpigeneticRegulatorsRevealsa RolefortheNuB4/HAT-BHistoneAcetyltransferase ComplexinHistoneTurnover KittyF.Verzijlbergen1.,TiborvanWelsem1.,DaoudSie2,3,TinekeL.Lenstra4,DanielJ.Turner5¤, FrankC.P.Holstege4,RonM.Kerkhoven2,3,FredvanLeeuwen1* 1Department of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands, 2Genome Center, Netherlands Cancer Institute, Amsterdam, The Netherlands,3NetherlandsProteomicsCenter,Amsterdam,TheNetherlands,4DepartmentofMolecularCancerResearch,UniversityMedicalCenterUtrecht,Utrecht,The Netherlands,5WellcomeTrustSangerInstitute,WellcomeTrustGenomeCampus,Hinxton,UnitedKingdom Abstract Dynamicmodificationofhistoneproteinsplaysakeyroleinregulatinggeneexpression.However,histonesthemselvescan alsobedynamic,whichpotentiallyaffectsthestabilityofhistonemodifications.Todeterminethemolecularmechanismsof histone turnover, we developed a parallel screening method for epigenetic regulators by analyzing chromatin states on DNA barcodes. Histone turnover was quantified by employing a genetic pulse-chase technique called RITE, which was combinedwithchromatinimmunoprecipitationandhigh-throughputsequencing.Inthisscreen,theNuB4/HAT-Bcomplex, containingtheconservedtypeBhistoneacetyltransferaseHat1,wasfoundtopromotehistoneturnover.Unexpectedly,the three members of this complex could be functionally separated from each other as well as from the known interacting factorandhistonechaperoneAsf1.Thus,systematicanddirectinterrogationofchromatinstructureonDNAbarcodescan leadtothediscoveryofgenesandpathwaysinvolvedinchromatinmodificationanddynamics. Citation:VerzijlbergenKF,vanWelsemT,SieD,LenstraTL,TurnerDJ,etal.(2011)ABarcodeScreenforEpigeneticRegulatorsRevealsaRolefortheNuB4/HAT-B HistoneAcetyltransferaseComplexinHistoneTurnover.PLoSGenet7(10):e1002284.doi:10.1371/journal.pgen.1002284 Editor:DirkSchu¨beler,FriedrichMiescherInstituteforBiomedicalResearch,Switzerland ReceivedFebruary24,2011;AcceptedJuly29,2011;PublishedOctober6,20