Altered Retinoic Acid Metabolism in Diabetic Mouse Kidney Identified by 18O Isotopic Labeling and 2D Mass Spectrometry 英文参考文献.docVIP

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Altered Retinoic Acid Metabolism in Diabetic Mouse Kidney Identified by 18O Isotopic Labeling and 2D Mass Spectrometry 英文参考文献.doc

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Altered Retinoic Acid Metabolism in Diabetic Mouse Kidney Identified by 18O Isotopic Labeling and 2D Mass Spectrometry 英文参考文献

AlteredRetinoicAcidMetabolisminDiabeticMouse KidneyIdentifiedby 18OIsotopicLabelingand2DMass Spectrometry JonathanM.Starkey2,YingxinZhao1,4,5,6,RovshanG.Sadygov2,6,SigmundJ.Haidacher1,5,WandaS. LeJeune1,5,NilayDey1,BruceA.Luxon2,6,7,MaureenA.Kane8¤,JosephL.Napoli8,LarryDenner1,4,5,6, RonaldG.Tilton1,3,4,5 * 1DepartmentofInternalMedicine,UniversityofTexasMedicalBranch,Galveston,Texas,UnitedStatesofAmerica,2DepartmentofBiochemistryandMolecularBiology, UniversityofTexasMedicalBranch,Galveston,Texas,UnitedStatesofAmerica,3DepartmentofOphthalmologyandVisualSciences,UniversityofTexasMedicalBranch, Galveston,Texas,UnitedStatesofAmerica,4StarkDiabetesCenter,UniversityofTexasMedicalBranch,Galveston,Texas,UnitedStatesofAmerica,5McCoyDiabetes MassSpectrometryResearchLaboratory,UniversityofTexasMedicalBranch,Galveston,Texas,UnitedStatesofAmerica,6SealyCenterforMolecularMedicine,University of TexasMedical Branch,Galveston, Texas, United States ofAmerica,7Institute for Translational Science Biomedical Informatics Program,Universityof TexasMedical Branch, Galveston, Texas, United States ofAmerica, 8Department of Nutritional Scienceand Toxicology,University ofCalifornia, Berkeley, California, United States of America Abstract Background:Numerousmetabolicpathwayshavebeenimplicatedindiabetes-inducedrenalinjury,yetfewstudieshave utilizedunbiasedsystemsbiologyapproachesformappingtheinterconnectivityofdiabetes-dysregulatedproteinsthatare involved.Weutilizedaglobal,quantitative,differentialproteomicapproachtoidentifyanovelretinoicacidhubinrenal corticalproteinnetworksdysregulatedbytype2diabetes. Methodology/PrincipalFindings:Totalproteinswereextractedfromrenalcortexofcontrolanddb/dbmiceat20weeksof age (after 12 weeks of hyperglycemia in the diabetic mice). Following trypsinization, 18O- and 16O-labeled control and diabetic peptides, respectively, were pooled and separated by two dimensional liquid chromatography (strong cation exchangecreating60fractionsfurtherseparatedbynano-HPLC),fo