Amino Acid Transporter Genes Are Essential for FLO11-Dependent and FLO11-Independent Biofilm Formation and Invasive Growth in Saccharomyces cerevisiae 英文参考文献.docVIP
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Amino Acid Transporter Genes Are Essential for FLO11-Dependent and FLO11-Independent Biofilm Formation and Invasive Growth in Saccharomyces cerevisiae 英文参考文献
AminoAcidTransporterGenesAreEssentialforFLO11-
DependentandFLO11-IndependentBiofilmFormation
andInvasiveGrowthinSaccharomycescerevisiae
RasmusTorbensen1,HenrikDevittM?ller1,DavidGresham2,3,SefaAlizadeh1,DoreenOchmann4,
EckhardBoles4,BirgitteRegenberg1*
1Department of Biology, University of Copenhagen, Copenhagen, Denmark, 2Center for Genomics and Systems Biology, New York University, New York, New York,
United States ofAmerica, 3DepartmentofBiology, New York, New York University, New York, United States ofAmerica, 4Institute ofMolecular Biosciences, Goethe
UniversityFrankfurt,FrankfurtamMain,Germany
Abstract
AminoacidscaninduceyeastcelladhesionbuthowaminoacidsaresensedandsignalthemodulationoftheFLOadhesion
genesisnotclear.WediscoveredthatthebuddingyeastSaccharomycescerevisiaeCEN.PKevolvedinvasivegrowthability
underprolongednitrogenlimitation.Suchinvasivemutantswereusedtoidentifyaminoacidtransportersasregulatorsof
FLO11andinvasivegrowth.OneinvasivemutanthadelevatedlevelsofFLO11mRNAandaQ320STOPmutationintheSFL1
genethatencodesaproteinkinaseApathwayregulatedrepressorofFLO11.Glutamine-transportergenesDIP5andGNP1
wereessentialforFLO11expression,invasivegrowthandbiofilmformationinthismutant.Invasivegrowthreliedonknown
regulatorsofFLO11andtheSsy1-Ptr3-Ssy5complexthatcontrolsDIP5andGNP1,suggestingthatDip5andGnp1operates
downstreamoftheSsy1-Ptr3-Ssy5complexforregulationofFLO11expressioninaproteinkinaseAdependentmanner.The
role of Dip5 and Gnp1 appears to be conserved in the S. cerevisiae strain g1278b since the dip5 gnp1 g1278b mutant
showednoinvasivephenotype. Secondly,theaminoacidtransportergeneGAP1wasfoundtoinfluenceinvasivegrowth
throughFLO11aswellasotherFLOgenes.Cellscarryingadominantloss-of-functionPTR3647::CWNKNPLSSIN allelehadincreased
transcriptionoftheadhesiongenesFLO1,5,9,10,11andtheaminoacidtransportergeneGAP1.DeletionofGAP1caused
loss of FLO11 expression and invasive growth. However, deletions of FLO11 and genes encoding components of the
mitogen-activa
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