An Approach for Reliably Investigating Hippocampal Sharp Wave-Ripples In Vitro 英文参考文献.docVIP

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An Approach for Reliably Investigating Hippocampal Sharp Wave-Ripples In Vitro 英文参考文献.doc

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An Approach for Reliably Investigating Hippocampal Sharp Wave-Ripples In Vitro 英文参考文献

AnApproachforReliablyInvestigatingHippocampal SharpWave-RipplesInVitro NikolausMaier1,GenelaMorris1,2,3,FriedrichW.Johenning1,DietmarSchmitz1,3* 1Neuroscience Research Center, Charite′-Universita¨tsmedizin Berlin, Berlin, Germany, 2Department of Neurobiology and Ethology, Faculty of Science and Science Education,HaifaUniversity,Haifa,Israel,3BernsteinCenterforComputationalNeuroscience,HumboldtUniversityBerlin,Berlin,Germany Abstract Background: Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neuronsisessentialforcomprehensiveunderstandingofthenetworkfunctionduringcomplexactivitieslikeSPW-R,such knowledgeiscurrentlynotablyscarce. Methodology/Principal Findings: We demonstratean invitro approach toSPW-R thatoffers asimple experimentaltool allowing detailed analysis of mechanisms governing the sharp wave-state of the hippocampus. We combine interface storage of slices with modifications of a conventional submerged recording system and established in vitro SPW-R comparable to their in vivo counterpart. We show that slice storage in the interface chamber close to physiological temperatureistherequiredconditiontopreservenetworkintegritythatisnecessaryforthegenerationofSPW-R.Moreover, wedemonstratetheutilityofourmethodforstudyingsynapticandnetworkpropertiesofSPW-R,usingelectrophysiological andimagingmethodsthatcanonlybeappliedinthesubmergedsystem. Conclusions/Significance: The approach presented here demonstrates a reliable and experimentally simple strategy for studying hippocampal sharp wave-ripples. Given its utility and easy application we expect our model to foster the generationofnewinsightintothenetworkphysiologyunderlyingSPW-R. Citation:MaierN,MorrisG,JohenningFW,SchmitzD(2009)AnApproachforReliablyInvestigatingHippocampalSharpWave-RipplesInVitro.PLoSONE4(9): e6925.doi:10.1371/journal.pone.0006925 Editor