Antibody Phage Display Assisted Identification of Junction Plakoglobin as a Potential Biomarker for Atherosclerosis 英文参考文献.docVIP

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Antibody Phage Display Assisted Identification of Junction Plakoglobin as a Potential Biomarker for Atherosclerosis 英文参考文献.doc

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Antibody Phage Display Assisted Identification of Junction Plakoglobin as a Potential Biomarker for Atherosclerosis 英文参考文献

AntibodyPhageDisplayAssistedIdentificationof JunctionPlakoglobinasaPotentialBiomarkerfor Atherosclerosis SerainaCooksley-Decasper1,2,HansReiser1,10,DanielaS.Thommen3,BarbaraBiedermann3, MichelNeidhart2,4,JoannaGawinecka1,GieriCathomas5,FabianC.Franzeck6,ChristopheWyss6, RolandKlingenberg6,PaoloNanni7,BerndRoschitzki7,ChristianMatter2,6,PetraWolint8, MaximilianY.Emmert8,MarcHusmann9,BeatriceAmann-Vesti9,WilibaldMaier2,6,SteffenGay2,4 ThomasF.Lu¨scher2,6,ArnoldvonEckardstein1,2,10*.,DanielleHof1. , 1Institute for Clinical Chemistry, University Hospital Zurich, Zurich, Switzerland, 2Zurich Center for Integrative Human Physiology (ZIHP), Zurich, Switzerland, 3Department of Biomedicine, University Hospital Basel, Basel, Switzerland, 4Institute for Experimental Rheumatology, University Hospital Zurich, Zurich, Zurich, Switzerland, 5Cantonal Institute for Pathology, Liestal, Switzerland, 6Department of Cardiology and Cardiovascular Physiology, University Hospital Zurich, Zurich, Switzerland,7FunctionalGenomicsCenterZurich(FGCZ),Zurich,Switzerland,8DepartmentofCardiovascularSurgery,UniversityHospitalZurich,Zurich,Switzerland, 9DepartmentofAngiology,UniversityHospitalZurich,Zurich,Switzerland,10CompetenceCenterforSystemsPhysiologyandMetabolicDiseases(CC-SPMD),Zurich, Switzerland Abstract To date, no plaque-derived blood biomarker is available to allow diagnosis, prognosis or monitoring of atherosclerotic vasculardiseases.Inthisstudy,specimensofthrombendarterectomymaterialfromcarotidandiliacarterieswereincubated inprotein-freemediumtoobtainplaqueandcontrolsecretomesforsubsequentsubtractivephagedisplay.Theselectionof nineplaquesecretome-specificantibodiesandtheanalysisoftheirimmunopurifiedantigensbymassspectrometryledto theidentificationof22proteins.Oneofthem,junctionplakoglobin(JUP-81)anditssmallerisoforms(referredtoasJUP-63, JUP-55andJUP-30bymolecularweight)wereconfirmedbyimmunohistochemistryandimmunoblottingwithindependent antibodiestobepresentinatheroscleroticplaquesandt