Antioxidant Biomarkers from Vanda coerulea Stems Reduce Irradiated HaCaT PGE-2 Production as a Result of COX-2 Inhibition 英文参考文献.docVIP

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Antioxidant Biomarkers from Vanda coerulea Stems Reduce Irradiated HaCaT PGE-2 Production as a Result of COX-2 Inhibition 英文参考文献.doc

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Antioxidant Biomarkers from Vanda coerulea Stems Reduce Irradiated HaCaT PGE-2 Production as a Result of COX-2 Inhibition 英文参考文献

AntioxidantBiomarkersfromVandacoeruleaStems ReduceIrradiatedHaCaTPGE-2ProductionasaResultof COX-2Inhibition CharlotteSimmler1,2*,CyrilAntheaume1,3,AnneliseLobstein1,2 1FacultyofPharmacy,UniversityofStrasbourg,Illkirch,France,2LaboratoiredePharmacognosie(UMR-CNRS7200),Illkirch,France,3ServiceCommund’Analyse(SCA)- RMN,Illkirch,France Abstract Background:InourinvestigationstowardstheisolationofpotentiallybiologicallyactiveconstituentsfromOrchidaceae,we carriedoutphytochemicalandbiologicalanalysesofVandaspecies.ApreliminarybiologicalscreeningrevealedthatVanda N coerulea(Griff.ex.Lindl)crudehydro-alcoholicstemextractdisplayedthebestDPPH/OHradicalscavengingactivityandin vitroinhibitionoftype2prostaglandin(PGE-2)releasefromUVB(60mJ/cm2)irradiatedHaCaTkeratinocytes. PrincipalFindings:Bio-guidedfractionationandphytochemicalanalysisledtotheisolationoffivestilbenoids:imbricatin(1) methoxycoelonin(2)gigantol(3)flavidin(4)andcoelonin(5).Stilbenoids(1–3)werethemostconcentratedincrudehydro- alcoholicstemextractandwereconsideredasVandacoeruleastembiomarkers.Dihydro-phenanthropyran(1)anddihydro- N phenanthrene (2) displayed the best DPPH/OH radical scavenging activities as well as HaCaT intracellular antioxidant properties(usingDCFH-DAprobe:IC508.8mMand9.4mM,respectively)comparedtobibenzyle(3)(IC5020.6mM).Inturn, thelattershowedaconstantinhibitionofPGE-2production,strongerthanstilbenoids(1)and(2)(IC 12.2mMand19.3mM, 50 respectively). Western blot analysis revealed that stilbenoids (1–3) inhibited COX-2 expression at 23mM. Interestingly, stilbenoids(1)and(2)butnot(3)wereabletoinhibithumanrecombinantCOX-2activity. Conclusions:Majorantioxidantstilbenoids(1–3)fromVandacoeruleastemsdisplayedaninhibitionofUVB-inducedCOX-2 expression.Imbricatin(1)andmethoxycoelonin(2)werealsoabletoinhibitCOX-2activityinaconcentration-dependent mannertherebyreducingPGE-2productionfromirradiatedHaCaTcells.Ourstudiessuggestthatstilbenoids(1–3)couldbe potentiallyusedforskinprotectionagainstthedamageca