ATM Limits Incorrect End Utilization during Non-Homologous End Joining of Multiple Chromosome Breaks 英文参考文献.docVIP

下载本文档

3
0
约6.17万字
约 11页
2017-05-11 发布于上海
举报
版权申诉

ATM Limits Incorrect End Utilization during Non-Homologous End Joining of Multiple Chromosome Breaks 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

ATM Limits Incorrect End Utilization during Non-Homologous End Joining of Multiple Chromosome Breaks 英文参考文献

ATMLimitsIncorrectEndUtilizationduringNon- HomologousEndJoiningofMultipleChromosome Breaks NicoleBennardo,JeremyM.Stark* DepartmentofCancerBiologyandIrellandManellaGraduateSchoolofBiologicalSciences,BeckmanResearchInstituteoftheCityofHope,Duarte,California,United StatesofAmerica Abstract Chromosome rearrangements can form when incorrect ends are matched during end joining (EJ) repair of multiple chromosomal double-strand breaks (DSBs). We tested whether the ATM kinase limits chromosome rearrangements via suppressingincorrectendutilizationduringEJrepairofmultipleDSBs.Forthis,wedevelopedasystemformonitoringEJof twotandemDSBsthatcanberepairedusingcorrectends(Proximal-EJ)orincorrectends(Distal-EJ,whichcauseslossofthe DNAbetweentheDSBs).Inthissystem,twoDSBsareinducedinachromosomalreporterbythemeganucleaseI-SceI.These DSBsareprocessedintonon-cohesiveendsbytheexonucleaseTrex2,whichleadstotheformationofI-SceI–resistantEJ products during both Proximal-EJ and Distal-EJ. Using this method, we find that genetic or chemical disruption of ATM causesasubstantial increase in Distal-EJ,but not Proximal-EJ.We also find that theincrease inDistal-EJ caused by ATM disruptionisdependentonclassicalnon-homologousendjoining(c-NHEJ)factors,specificallyDNA-PKcs,Xrcc4,andXLF. WepresentevidencethatNbs1-deficiencyalsocauseselevatedDistal-EJ,butnotProximal-EJ,toasimilardegreeasATM- deficiency.Inaddition,toevaluatetherolesofthesefactorsonendprocessing,weexaminedDistal-EJrepairjunctions.We foundthatATMandXrcc4limitthelengthofdeletions,whereasNbs1andDNA-PKcspromoteshortdeletions.Thus,the regulationofendprocessingappearsdistinctfromthatofendutilization.Insummary,wesuggestthatATMisimportantto limitincorrectendutilizationduringc-NHEJ. Citation: Bennardo N, Stark JM (2010) ATM Limits Incorrect End Utilization during Non-Homologous End Joining of Multiple Chromosome Breaks. PLoS Genet6(11):e1001194.doi:10.1371/journal.pgen.1001194 Editor:NancyMaizels,UniversityofWashington,UnitedStatesofAmerica Receiv