Bub1 Kinase Targets Sgo1 to Ensure Efficient Chromosome Biorientation in Budding Yeast Mitosis 英文参考文献.docVIP

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Bub1 Kinase Targets Sgo1 to Ensure Efficient Chromosome Biorientation in Budding Yeast Mitosis 英文参考文献.doc

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Bub1 Kinase Targets Sgo1 to Ensure Efficient Chromosome Biorientation in Budding Yeast Mitosis 英文参考文献

Bub1KinaseTargetsSgo1toEnsure EfficientChromosomeBiorientation inBuddingYeastMitosis Josefin Fernius, Kevin G. Hardwick* WellcomeTrustCentreforCellBiology,InstituteofCellBiology,UniversityofEdinburgh,UnitedKingdom Duringcelldivisionallchromosomesmustbesegregatedaccuratelytoeachdaughtercell.Errorsinthisprocessgive risetoaneuploidy,whichleadstobirthdefectsandisimplicatedincancerprogression.Thespindlecheckpointisa surveillance mechanism that ensures high fidelity of chromosome segregation by inhibiting anaphase until all kinetochoreshaveestablishedbipolarattachmentstospindlemicrotubules.Bub1kinaseisacorecomponentofthe spindlecheckpoint,andcellslackingBub1failtoarrestinresponsetomicrotubuledrugsandprecociouslysegregate their DNA. The mitotic role(s) of Bub1 kinase activity remain elusive,and it is controversial whether this C-terminal domainofBub1pisrequiredforspindlecheckpointarrest.Herewemakeadetailedanalysisofbuddingyeastcells lacking the kinase domain (bub1DK). We show that despite being able to arrest in response to microtubule depolymerisationandkinetochore-microtubuleattachmentdefects,bub1DKcellsaresensitivetomicrotubuledrugs. This is because bub1DK cells display significant chromosome mis-segregation upon release from nocodazole arrest. bub1DKcellsmislocaliseSgo1p,andwedemonstratethatboththeBub1kinasedomainandSgo1parerequiredfor accuratechromosomebiorientationafternocodazoletreatment.WeproposethatBub1kinaseandSgo1pacttogether toensureefficientbiorientationofsisterchromatidsduringmitosis. Citation:FerniusJ,HardwickKG(2007)Bub1kinasetargetsSgo1toensureefficientchromosomebiorientationinbuddingyeastmitosis.PLoSGenet3(11):e213.doi:10.1371/ journal.pgen.0030213 chores [19,20] and to lack of tension across sister kineto- chores that have yet to achieve proper biorientation [21]. Introduction The?delityofchromosomesegregationisdependentupon correctbipolarattachmentofsisterchromatidstothespindle microtubules (for review see [1]). These attachments are mediated through