Burkholderia thailandensis Is Virulent in Drosophila melanogaster 英文参考文献.docVIP

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Burkholderia thailandensis Is Virulent in Drosophila melanogaster 英文参考文献.doc

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Burkholderia thailandensis Is Virulent in Drosophila melanogaster 英文参考文献

BurkholderiathailandensisIsVirulentinDrosophila melanogaster MartinaPila′tova′1,2¤,MarcS.Dionne2* 1DepartmentofCraniofacialDevelopment,DentalInstitute,SchoolofMedicine,King’sCollegeLondon,London,UnitedKingdom,2CentreforMolecularandCellular BiologyofInflammation,DepartmentofImmunobiology,DIIID,SchoolofMedicine,King’sCollegeLondon,London,UnitedKingdom Abstract MelioidosisisaseriousinfectiousdiseaseendemictoSoutheastAsiaandNorthernAustralia.Thisdiseaseiscausedbythe Gram-negativebacteriumBurkholderiapseudomallei;Burkholderiathailandensisisaclosely-relatedorganismknowntobe avirulent in humans. B. thailandensis has not previously been used to infect Drosophila melanogaster. We examined the effectofB.thailandensisinfectiononflysurvival,onantimicrobialpeptideexpression,andonphagocyticcells.Inthefruit fly,whichpossessesonlyaninnateimmunesystem,B.thailandensisishighlyvirulent,causingrapiddeathwheninjectedor fed.Oneintriguingaspectofthisinfectionisitstemperaturedependence:infectedfliesmaintainedat25uCexhibitrapid bacterial proliferation and death in a few days, while infected animals maintained at 18uC exhibit very slow bacterial proliferationandtakeweekstodie;thiseffectis dueinparttodifferencesinimmuneactivityofthehost. Deathinthis infectionislikelydueatleastinparttoasecretedtoxin,asinjectionofflieswithsterileB.thailandensis-conditionedmedium isabletokill.B.thailandensisinfectionstronglyinducestheexpressionofantimicrobialpeptides,butthisisinsufficientto inhibit bacterial proliferation in infected flies. Finally, the function of fly phagocytes is not affected by B. thailandensis infection.ThehighvirulenceofB.thailandensisintheflysuggeststhepossibilitythatthisorganismisanaturalpathogenof oneormoreinvertebrates. Citation: M, Dionne MS (2012) Burkholderia thailandensis Is Virulent in Drosophila melanogaster. PLoS ONE 7(11): e49745. doi:10.1371/ Pila′tova′ journal.pone.0049745 Editor:Franc?ois Leulier,EcoleNormaleSupe′rieur deLyon,France ReceivedApril13,201