华中科技大学同济医学院免疫学系.pdfVIP

邱文洪等　hIL10 修饰树突状细胞对实验动物淋巴细胞增殖及胞毒效应的影响　第 8 期 5·27 · hIL10 修饰树突状细胞对实验动物淋巴细胞增殖及胞毒效应的影响① ② ③ 邱文洪 　郭凯文 　朱慧芬 　廖雯君 　王国华 　沈关心 (华中科技大学同济医学院免疫学系 ,武汉 430030) 　　中国图书分类号 　R392. 4 　　文献标识码 　A 　　文章编号 　1000484X( 2003) [摘 　要] 　目的 :探讨 hIL10 修饰 DC 对实验动物免疫功能的影响。方法 :将经 hIL10 基因修饰或未修饰 DC 腹腔注射 C57BL6 致敏小鼠 , 以致敏或未致敏 C57BL6 单个核细胞作为反应细胞 , 以未修饰DC 细胞及修饰 DC 为刺激细胞 ,共培养 6 天 ,MTT 检测细胞增殖 ,乳酸脱氢酶法测定细胞毒活性。结果 :hIL10 对未修饰 DC 致敏或未致敏小鼠的同种细胞刺激的增殖 反应有明显的抑制作用。hIL10 修饰 DC 诱导不同组小鼠淋巴细胞增殖反应显著低于未修饰 DC 细胞诱导小鼠淋巴细胞增殖 反应 , hIL10 修饰 DC 对不同组小鼠 CTL 细胞毒活性具有抵抗作用。结论 :hIL10 修饰的 DC 诱导同种小鼠淋巴细胞的增殖反 应显著降低和对 CTL 胞毒活性抵抗。 [ 关键词] 　树突状细胞 ; IL10 ;单向混合淋巴细胞培养 ;乳酸脱氢酶法 The influence of IL10modified dendritic cells on proliferation and cytotoxicity of lymphocytes in experimental animals QIU WenHong , GUO KaiWen , ZHU HuiFen et al Institute of Immunology , Tongji Medical College , Huazhong Uni versity of Science and Technology , Wuhan 430030 , China [ Abstract ] 　Objective :To investigate the influence of dendritic cells modified by hIL10 on proliferation and cytotoxicity of lymphocyte in experimental animalsMethods : The modified dendritic cells were injected intraperitoneally into C57BL6 mice to sensitize it Peripheral blood mononuclear cells (PBMC) of sensitized or unsensitized C57BL6 mice act as reactive cells , dendritic cells modified or unmodified by hIL10 act as stimulation cells These two type cells cocultured for 6 days MTT assay was used to detect lymphocyte proliferation Lactate de hydrogenase release method was used to examine the cytotoxic activityResults :The results showed that IL10 can inhibit lymphocyte prolifera tion reaction of unsensitized or sensitized C57BL6 mice Lymphocyte proliferation induced by modified dendritic cells is significant lower than that by unmodified dendritic cells Mod