Development of a Foot-and-Mouth Disease Virus Serotype A Empty Capsid Subunit Vaccine Using Silkworm (Bombyx mori) Pupae 英文参考文献.docVIP

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Development of a Foot-and-Mouth Disease Virus Serotype A Empty Capsid Subunit Vaccine Using Silkworm (Bombyx mori) Pupae 英文参考文献.doc

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Development of a Foot-and-Mouth Disease Virus Serotype A Empty Capsid Subunit Vaccine Using Silkworm (Bombyx mori) Pupae 英文参考文献

DevelopmentofaFoot-and-MouthDiseaseVirus SerotypeAEmptyCapsidSubunitVaccineUsing Silkworm(Bombyxmori)Pupae ZhiyongLi1,YongzhuYi2,XiangpingYin1,YunZhang1,MingLiu1,HangLiu1,XueruiLi1,Yinu¨ Li2, ZhifangZhang2*,JixingLiu1* 1State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, ChineseAcademyofAgricultureSciences,Lanzhou,Gansu,China,2BiotechnologyResearchInstitute,ChineseAcademyofAgriculturalSciences,Beijing,China Abstract Foot-and-mouthdisease(FMD)isahighlycontagiousdiseaseofcloven-hoofedanimalsthatinflictssevereeconomiclosses inthelivestockindustry.In2009,FMDVserotypeAcausedoutbreaksofFMDincattleinChina.Althoughaninactivated virusvaccinehasproveneffectivetocontrolFMD,itsusemayleadtonewdiseaseoutbreaksduetoapossibleincomplete inactivationofthevirusduringthemanufacturingprocess.Here,weexpressedtheP1-2Aandthe3Ccodingregionsofa serotype A FMDV field isolate in silkworm pupae (Bombyx mori) and evaluated the immunogenicity of the expression products. Four of five cattle vaccinated with these proteins developed high titers of FMDV-specific antibody and were completely protected against virulent homologous virus challenge with 10,000 50% bovine infectious doses (BID50). Furthermore,the50%bovineprotectivedose(PD50)testwasperformedtoassessthebovinepotencyoftheemptycapsid subunitvaccineandwasshowntoachieve4.33PD50perdose.Thesedataprovideevidencethatsilkwormpupaecanbe usedtoexpressimmunogenicFMDVproteins.Thisstrategymightbeusedtodevelopanewgenerationofemptycapsid subunitvaccinesagainstavarietyofdiseases. Citation:LiZ,YiY,YinX,ZhangY,LiuM,etal.(2012)DevelopmentofaFoot-and-MouthDiseaseVirusSerotypeAEmptyCapsidSubunitVaccineUsingSilkworm (Bombyxmori)Pupae.PLoSONE7(8):e43849.doi:10.1371/journal.pone.0043849 Editor:DingXiangLiu,NanyangTechnologicalUniversity,Singapore ReceivedOctober13,2010;AcceptedJuly30,2012;PublishedAugust27,2012 Copyright:?2012Lietal.Thisisanopen-a