Differences in the Properties and Mirna Expression Profiles between Side Populations from Hepatic Cancer Cells and Normal Liver Cells 英文参考文献.docVIP

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Differences in the Properties and Mirna Expression Profiles between Side Populations from Hepatic Cancer Cells and Normal Liver Cells 英文参考文献.doc

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Differences in the Properties and Mirna Expression Profiles between Side Populations from Hepatic Cancer Cells and Normal Liver Cells 英文参考文献

DifferencesinthePropertiesandMirnaExpression ProfilesbetweenSidePopulationsfromHepaticCancer CellsandNormalLiverCells Wei-huiLiu1,2.,Kai-shanTao2.,NanYou2.,Zheng-caiLiu2,Hong-taoZhang2,Ke-fengDou2* 1PLACenterofGeneralSurgery,GeneralHospitalofChengduArmyRegion,Chengdu,SichuanProvince,China,2DepartmentofHepatobiliarySurgery,XijingHospital, FourthMilitaryMedicalUniversity,Xi’an,ShaanxiProvince,China Abstract Aims:Becausehepaticcancerstemcells(HCSCs)arebelievedtoderivefromtheconversionofhepaticnormalstemcells (HNSCs),theidentificationofthedifferencesthatdistinguishHCSCsfromHNSCsisimportant. Methods:TheHCCmodelwasestablishedinF344ratsbyDENinduction.UsingFACSanalysis,sidepopulationcellsfrom HCC(SP-HCCs)wereisolatedfromtheepithelial-likecellsofHCCtissues,andthesidepopulationcellsfromnormalliver(SP- NLCs) were isolated from syngeneic normal liver cells. The expression of stem cell markers was detected in both freshly isolatedandamplifiedsubpopulations.AfterinductionwithHGF,thedifferentiationofeachsubpopulationwasanalyzedby detectionofearlyandlatelivermarkers.Invivo,thebiologicalcharacteristicsofSP-HCCsandSP-NLCswereanalyzedby repairing injured livers or forming tumors in nude mice. In addition, the expression of miRNAs was examined in both populationsbymiRNAarrayandQRT-PCR. Results:SP-NLCsandSP-HCCswere4.3060.011%and2.10060.010%ofthewholepopulation,respectively.BothSP-NLCs and SP-HCCs displayed greater expression of stem cell markers (CD133 and EpCAM) than NSP-NLCs and NSP-HCCs, respectively (P,0.01), both after fresh isolation and amplification. Upon HGF induction, SP-NLCs generated many ALB positivecellsandfewCK-7positivecells,butNSP-NLCscouldgenerateonlyALBpositivecells.Incontrast,SP-HCCsgaverise toonlyAFPpositivecells.Asfewas56105SP-NLCswerecapableofrepairingliverinjury,whilethesamenumberofNSP- NLCscouldnotrepairtheliver.Furthermore,only1610 SP-HCCswerenecessarytoinitiateatumor,whileNSP-HCCscould not form a tumor. Compared to SP-NLCs, 68 up-regulated and 10 down-re