Differential Carbohydrate Recognition by Campylobacter jejuni Strain 11168 Influences of Temperature and Growth Conditions 英文参考文献.docVIP

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Differential Carbohydrate Recognition by Campylobacter jejuni Strain 11168 Influences of Temperature and Growth Conditions 英文参考文献.doc

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Differential Carbohydrate Recognition by Campylobacter jejuni Strain 11168 Influences of Temperature and Growth Conditions 英文参考文献

DifferentialCarbohydrateRecognitionbyCampylobacter jejuniStrain11168:InfluencesofTemperatureand GrowthConditions ChristopherJ.Day,JoeTiralongo*,ReganD.Hartnell,Carie-AnneLogue,JenniferC.Wilson,Markvon Itzstein,VictoriaKorolik* InstituteforGlycomics,GriffithUniversityGoldCoastCampus,Queensland,Australia Abstract ThepathogenicclinicalstrainNCTC11168wasthefirstCampylobacterjejunistraintobesequencedandhasbeenawidely usedlaboratorymodelforstudyingC.jejunipathogenesis.However,continuouspassagingofC.jejuniNCTC11168hasbeen showntodramaticallyaffectitscolonisationpotential.GlycanarrayanalysiswasperformedonC.jejuniNCTC11168using thefrequentlypassaged,non-colonising,genomesequenced(11168-GS)andtheinfrequentlypassaged,original,virulent (11168-O) isolates grown or maintained under various conditions. Glycan structures recognised and bound by C. jejuni included terminal mannose, N-acetylneuraminic acid, galactose and fucose. Significantly, it was found that only when challenged with normal oxygen at room temperature did 11168-O consistently bind to sialic acid or terminal mannose structures,while11168-GSboundthesestructuresregardlessofgrowth/maintenanceconditions.Further,bindingofun- capped galactose and fucosylated structures was significantly reduced when C. jejuni was maintained at 25uC under atmosphericoxygenconditions.Thesebindingdifferencesidentifiedthroughglycanarrayanalysiswereconfirmedbythe abilityofspecificlectinstocompetitivelyinhibittheadherenceofC.jejunitoaCaco-2intestinalcellline.Ourdatasuggests thatthebindingofmannoseand/orN-acetylneuraminicacidmayprovidetheinitialinteractionsimportantforcolonisation followingenvironmentalexposure. Citation: Day CJ, Tiralongo J, Hartnell RD, Logue C-A, Wilson JC, et al. (2009) Differential Carbohydrate Recognition by Campylobacter jejuni Strain 11168: InfluencesofTemperatureandGrowthConditions.PLoSONE4(3):e4927.doi:10.1371/journal.pone.0004927 Editor:MarkusM.Heimesaat,Charite′,CampusBenjaminFranklin,Germany ReceivedNovember11,200