Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout 英文参考文献.docVIP

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Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout 英文参考文献.doc

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Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout 英文参考文献

DifferentialConservationandDivergenceofFertility GenesbouleanddazlintheRainbowTrout MingyouLi1.,QianShen1.,HongyanXu1,FoongMeiWong1,JianzhouCui1,ZhendongLi1,NiHong1, LiWang1,HaobinZhao1,BoMa2,YunhanHong1,3* 1DepartmentofBiologicalSciences,NationalUniversityofSingapore,Singapore,Singapore,2HeilongjiangFisheriesResearchInstitute,Ha’erbinCity,China,3Yangtze RiverFisheriesResearchInstitute,ChineseAcademyofFisherySciences,Jingzhou,China Abstract Background: The genes boule anddazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteinsessentialforgermcelldevelopment.Althoughdazlexhibitsbisexualexpressioninmitoticandmeioticgermcellsin diverseanimals,bouleshowsunisexualmeioticexpressionininvertebratesandmammalsbutabisexualmitoticandmeiotic expressioninmedaka.Howbouleanddazlhaveevolveddifferentexpressionpatternsindiverseorganismshasremained unknown. Methodology and Principal Findings: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identifiedcDNAsencodingthetroutBouleandDazlproteins,whichhaveaconservedRNA-recognitionmotifandamaximal similaritytotheirhomologs.ByRT-PCRanalysis,adultRNAexpressionoftroutbouleanddazlisrestrictedtothegonadsof bothsexes. Bychromogenicandtwo-colorfluorescence insituhybridization, werevealedbisexualandgermline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentratesintheBalbinani’sbodyofearlyoocytesandthechromatoidbodyofsperm.Surprisingly,bouleexhibitsmitotic andmeioticexpressioninthemalebutmeiosis-specificexpressioninthefemale. Conclusions: Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution.Weproposeamodelinwhichthediversityofbouleexpressioninsexandstagespecificitymighthaveresulted fromselectivelossorgainofitsexpressioninonesexandmitoticgermcel