Differential Localization and Independent Acquisition of the H3K9me2 and H3K9me3 Chromatin Modifications in the Caenorhabditis elegans Adult Germ Line 英文参考文献.docVIP

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Differential Localization and Independent Acquisition of the H3K9me2 and H3K9me3 Chromatin Modifications in the Caenorhabditis elegans Adult Germ Line 英文参考文献.doc

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Differential Localization and Independent Acquisition of the H3K9me2 and H3K9me3 Chromatin Modifications in the Caenorhabditis elegans Adult Germ Line 英文参考文献

DifferentialLocalizationandIndependentAcquisitionof theH3K9me2andH3K9me3ChromatinModificationsin theCaenorhabditiselegansAdultGermLine JessicaB.Bessler1,ErikC.Andersen2,AnneM.Villeneuve1* 1Departments of Developmental Biology and Genetics, Stanford University, Stanford, California, United States of America, 2Department of Biology, Howard Hughes MedicalInstitute,MassachusettsInstituteofTechnology,Cambridge,Massachusetts,UnitedStatesofAmerica Abstract Histone methylation is a prominent feature of eukaryotic chromatin that modulates multiple aspects of chromosome function.Methylmodificationcanoccuronseveraldifferentaminoacidresiduesandindistinctmono-,di-,andtri-methyl states. However, the interplay among these distinct modification states is not well understood. Here we investigate the relationshipsbetweendimethylandtrimethylmodificationsonlysine9ofhistoneH3(H3K9me2andH3K9me3)intheadult Caenorhabditis elegans germ line. Simultaneous immunofluorescence reveals very different temporal/spatial localization patternsforH3K9me2andH3K9me3.WhileH3K9me2isenrichedonunpairedsexchromosomesandundergoesdynamic changesasgermcellsprogressthroughmeioticprophase,wedemonstrateherethatH3K9me3isnotenrichedonunpaired sex chromosomes and localizes to all chromosomes in all germ cells in adult hermaphrodites and until the primary spermatocyte stage in males. Moreover, high-copy transgene arrays carrying somatic-cell specific promoters are highly enrichedforH3K9me3(butnotH3K9me2)andcorrelatewithDAPI-faintchromatindomains.Wefurtherdemonstratethat theH3K9me2andH3K9me3marksareacquiredindependently.MET-2,amemberoftheSETDBhistonemethyltransferase (HMTase) family, is required for all detectable germline H3K9me2 but is dispensable for H3K9me3 in adult germ cells. Conversely,weshowthattheHMTaseMES-2,anE(z)homologresponsibleforH3K27methylationinadultgermcells,is required for much of the germline H3K9me3 but is dispensable for H3K9me2. Phenotypic analysis of met-2 mutants indicatesthatMET-2isnonessen