Differential Transcription of Bacteriophage φX174 Genes at 37°C and 42°C 英文参考文献.docVIP

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Differential Transcription of Bacteriophage φX174 Genes at 37°C and 42°C 英文参考文献.doc

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Differential Transcription of Bacteriophage φX174 Genes at 37°C and 42°C 英文参考文献

DifferentialTranscriptionofBacteriophagewX174Genes 6 6 at37 Cand42 C LuyiZhao,AmberD.Stancik,CelesteJ.Brown* DepartmentofBiologicalSciences,UniversityofIdaho,Moscow,Idaho,UnitedStatesofAmerica Abstract Toinvestigatehowhightemperatureaffectsviraltranscription,theabsoluteamountsofmRNAforsixbacteriophagewX174 geneswere compared at 37uC and42uCusing Q-PCR. At37uC,mRNA levels for allgenes wereconsistent with previous studies,butat42uCmRNAlevelsforfourgenesweresignificantlydifferentfromlevelsat37uC.Transcriptlevelswerehigher forgenesBandD;thepromoterbeforegeneBappearstobeup-regulatedathightemperature.LevelsforgenesFandG werereducedathightemperature,possiblyduetoincreasedefficiencyofthetranscriptionterminationsignalimmediately upstream of gene F. These functional changes in wX174 gene regulation at high temperature have not been described previously.Studiesofphageevolutionathightemperaturesindicatethatthisdifferenceintranscriptlevelsissubjectto adaptation. Citation:ZhaoL,StancikAD,BrownCJ(2012)DifferentialTranscriptionofBacteriophagewX174Genesat37uCand42uC.PLoSONE7(4):e35909.doi:10.1371/ journal.pone.0035909 Editor:BaochuanLin,NavalResearchLaboratory,UnitedStatesofAmerica ReceivedJanuary5,2012;AcceptedMarch26,2012;PublishedApril23,2012 Copyright: ? 2012 Zhao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was supported by National Institutes of Health () grant NCRR P20RR16448. The funder had no role in study design, data collectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:celesteb@ Introduction providing new information about cis-regulatory regions of this well-studiedphage. During the latter half of the twentieth century, the bacterio- phagewX174wasusedasamodelorganismfort